Goat anti-Mouse TFEC Polyclonal Antibody

TFEC, ID (Transcription Factor EC, TFE-C, Class E Basic Helix-loop-helix Protein 34, bHLHe34, Transcription Factor EC-like, hTFEC-L, BHLHE34, TCFEC, TFECL)

Cat. Num. AAA6011141

• Reactivity: Mouse
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TFEC; Polyclonal Antibody; ID (Transcription Factor EC; TFE-C; Class E Basic Helix-loop-helix Protein 34; bHLHe34; Transcription Factor EC-like; hTFEC-L; BHLHE34; TCFEC; TFECL); Anti -TFEC; anti-TFEC antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Recognizes mouse TFEC. Species sequence homology: Rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-TFEC antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to C-HITKQQTHPERN from an internal region of mouse TFEC.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Spleen (formalin-fixed, paraffin-embedded) stained with TFEC antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-TFEC antibody

Expression of the mouse transcription factor EC (Tfec) is restricted to the myeloid compartment, suggesting a function for Tfec in the development or function of these cells. However, mice lacking Tfec develop normally, indicating a redundant role for Tfec in myeloid cell development. It is reported that Tfec is specifically induced in bone marrow-derived macrophages upon stimulation with the Th2 cytokines, IL-4 and IL-13, or LPS. LPS induced a rapid and transient up-regulation of Tfec mRNA expression and promoter activity, which was dependent on a functional NF-kappaB site. IL-4, however, induced a rapid, but long-lasting, increase in Tfec mRNA, which, in contrast to LPS stimulation, also resulted in detectable levels of Tfec protein. IL-4-induced transcription of Tfec was absent in macrophages lacking Stat6, and its promoter depended on two functional Stat6-binding sites. A global comparison of IL-4-induced genes in both wild-type and Tfec mutant macrophages revealed a surprisingly mild phenotype with only a few genes affected by Tfec deficiency. These included the G-CSFR (Csf3r) gene that was strongly up-regulated by IL-4 in wild-type macrophages and, to a lesser extent, in Tfec mutant macrophages.

Product Categories/Family for anti-TFEC antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 2347003
• NCBI Official Full Name: TFEC isoform (or TFECL)
• Protein Family: Transcription factor

Uniprot Description

TFEC: Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter. Collaborates with MITF in target gene activation. Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence. Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer. Binds DNA in a homo- or heterodimeric form. Belongs to the MiT/TFE family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein dimerization activity; DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent