Mouse anti-Mouse, Rat MHC Class 2, I-Ak/s Monoclonal Antibody

MHC Class 2, I-Ak/s (MHC Class II) (APC)

Cat. Num. AAA6269000

• Reactivity: Mouse, Rat
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: MHC Class 2; I-Ak/s; Monoclonal Antibody; I-Ak/s (MHC Class II) (APC); anti-MHC Class 2; I-Ak/s antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10F125
• Specificity: Recognizes a monomorphic determinant of the rat MHC class II I-Ak/s antigen present on B lymphocytes, dendric cells, some macrophages and certain epithelial cells. Does not react with the rat BDIX strain due to a defect in RT1B expression. Crossreacts with with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Species Crossreactivity: mouse
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MHC Class 2, I-Ak/s antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Immunogen: Rat thymocyte membrane glycoprotein
• Conjugate: APC
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Immunohistochemistry (IHC)

(M3887-54A used for the identification of MHC class II expressing microglial cells in rat brain by immunohistochemistry on formalin fixed free foating sections. MHC II+cells in the corpus callosum (CC) after tMCAO. Temporal expression of MHC II in the CC contralateral and ipsilateral to the lesioned hemisphere at different time points after tMCAO and a sham operated animal, respectively. Scale bars: lower magnification - 100uM; higher magnification - 20uM. From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment..)

Immunohistochemistry (IHC)

(M3887-54A used for the identification of activated microglia in rat brain by immunohistochemistry on formalin fixed free-floating sections. Spatiotemporal expression of MHC II protein in the ischemic hemisphere. Spatiotemporal distribution of MHC II expressing cells in the ipsilateral cortex of a sham operated animal and at the indicated time points after tMCAO. The border of the infarct core is delineated by a dotted line. Scale bars: low magnification - 200um; higher magnification - 20um. Abbreviations: V, lateral ventricle; IC, infarct core.)

Testing Data

(Staining of rat spleen cells withMBS607599.)

Testing Data

(A488 conjugated mouse anti rat CD45RA and A647 conjugated mouse IgG1 isotype control. Figure B. A488 conjugated mouse anti rat CD45RA and A647 conjugated mouse anti rat MHC-II. All experiments performed on red cell lysed rat blood gated on mononuclear cells.)

Product Categories/Family for anti-MHC Class 2, I-Ak/s antibody

Antibodies; MHC

References

1. McMaster, W. R. and Williams, A. F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur. J. Immunol. 9: 426-433. 2. Fernandez, J. L. and Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens. Lab. Anim. 20: 293-297. 3. Charteris, D. G. and Lightman, S. L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis. Immunology. 78: 387-392. 4. Whiteland, J. L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320. 5. McKechnie, N. M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia. J. Infect. Dis. 176: 1334-1343. 6. Male, D. K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strain J. Immunogenet. 14: 301-312. 7. Meyer zu Horste, G. et al. (2011) Quinpramine ameliorates rat experimental autoimmune neuritis and redistributes MHC class II molecules. PLoS One. 6(6): e21223.