Rabbit anti-Mouse FEN1 Polyclonal Antibody | anti-FEN1 antibody

RABBIT ANTI HUMAN FEN1

Cat. Num. AAA221739

• Gene Names: FEN1; MF1; RAD2; FEN-1
• Reactivity: Mouse
• Applications: Immunoprecipitation, Western Blot
• Synonyms: FEN1; Polyclonal Antibody; RABBIT ANTI HUMAN FEN1; anti-FEN1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 380

• Applicable Applications for anti-FEN1 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 42kD in HeLa cell lysates.; Immunoprecipitation: Minimum Dilution: 4ug/mg protein; Maximum Dilution: 10ug/mg protein; Western Blotting: Minimum Dilution: 1/5000; Maximum Dilution: 1/30000
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Buffer Solution: TRIS-citrate/Phosphate buffered saline
• Antiserum Preparation: Antisera to human FEN1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Detection of human Fen1 in whole cell lysates of HeLa and 293T cells (5-50 mcg/lane) by Western blotting with Rabbit anti Human Fen1 (MBS221739). Immunoprecipitation was with MBS221739 (3mcg/mg lysate) or another anti Fen1 polyclonal antibody, subsequent detection of immunoprecipitated Fen1 by Western blotting used MBS221739)

Related Product Information for anti-FEN1 antibody

Rabbit anti Human FEN1 antibody recognizes human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45kDa member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair (Kim et al. 1998). It is hypothesised that FEN1 may play a role in the inhibltion of triplet repeat expansion (Vallur and Maizels 2010). Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against which rabbit anti human FEN1 antibody is generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAase activity (Kazak et al. 2013). Initial results suggest that rabbit anti human FEN1 antibody does not recognize the truncated form of human FEN1. Fen1 is expressed in the nucleus of a wide variety of organ strusctures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Rabbit anti human FEN1 antibody recognizes a band of ~45kDa in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.

NCBI and Uniprot Product Information

• NCBI GI #: 4758356
• NCBI GeneID: 2237
• NCBI Accession #: NP_004102.1
• NCBI GenBank Nucleotide #: NM_004111.5
• UniProt Accession #: P39748; AAB32522
• Molecular Weight: 35,673 Da
• NCBI Official Full Name: flap endonuclease 1
• NCBI Official Synonym Full Names: flap structure-specific endonuclease 1
• NCBI Official Symbol: FEN1
• NCBI Official Synonym Symbols: MF1; RAD2; FEN-1
• NCBI Protein Information: flap endonuclease 1; DNase IV; FEN-1; FEN1; maturation factor 1; maturation factor-1
• UniProt Protein Name: Flap endonuclease 1
• UniProt Gene Name: FEN1
• UniProt Entry Name: FEN1_HUMAN

NCBI Description

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Uniprot Description

FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein type: DNA-binding; Nuclear receptor co-regulator; EC 3.1.-.-; DNA repair, damage; Ribonuclease; Nucleolus; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: nucleoplasm; mitochondrion; membrane; plasma membrane; nucleolus; nucleus

Molecular Function: 5'-3' exonuclease activity; protein binding; DNA binding; 5'-flap endonuclease activity; endonuclease activity; manganese ion binding; exonuclease activity; double-stranded DNA binding; damaged DNA binding; magnesium ion binding; ribonuclease H activity; double-stranded DNA specific exodeoxyribonuclease activity

Biological Process: DNA replication, removal of RNA primer; DNA strand elongation during DNA replication; DNA repair; DNA catabolic process, endonucleolytic; memory; telomere maintenance via semi-conservative replication; UV protection; base-excision repair; double-strand break repair; telomere maintenance via recombination; mitotic cell cycle; DNA replication; DNA catabolic process, exonucleolytic; telomere maintenance