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Immunohistochemistry (IHC)
(MBS607599 used for the identification of MHC class II expressing microglial cells in rat brain by immunohistochemistry on formalin fixed free foating sections. MHC II+cells in the corpus callosum (CC) after tMCAO. Temporal expression of MHC II in the CC contralateral and ipsilateral to the lesioned hemisphere at different time points after tMCAO and a sham operated animal, respectively. Scale bars: lower magnification - 100uM; higher magnification - 20uM. From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment..)
Mouse anti-Mouse, Rat MHC Class 2, I-Ak/s Monoclonal Antibody
MHC Class 2, I-Ak/s (MHC Class II)
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
MHC Class 2; I-Ak/s; Monoclonal Antibody; I-Ak/s (MHC Class II); Anti -MHC Class 2; anti-MHC Class 2; I-Ak/s antibody
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10F126
Specificity
Recognizes rat MHC class II I-Ak/s. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-MHC Class 2, I-Ak/s antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin).
Dilution: Flow Cytometry: 1:50-100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (paraffin): 1:50-1:100
Dilution: Flow Cytometry: 1:50-100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (paraffin): 1:50-1:100
Immunogen
Rat thymocyte membrane glycoprotein
Hybridoma
NS1 myeloma cells with spleen cells from BALB/c mice.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(MBS607599 used for the identification of MHC class II expressing microglial cells in rat brain by immunohistochemistry on formalin fixed free foating sections. MHC II+cells in the corpus callosum (CC) after tMCAO. Temporal expression of MHC II in the CC contralateral and ipsilateral to the lesioned hemisphere at different time points after tMCAO and a sham operated animal, respectively. Scale bars: lower magnification - 100uM; higher magnification - 20uM. From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment..)
Immunohistochemistry (IHC)
(MBS607599 used for the identification of MHC class II expressing microglial cells in rat brain by immunohistochemistry on formalin fixed free foating sections. MHC II+cells in the corpus callosum (CC) after tMCAO. Temporal expression of MHC II in the CC contralateral and ipsilateral to the lesioned hemisphere at different time points after tMCAO and a sham operated animal, respectively. Scale bars: lower magnification - 100uM; higher magnification - 20uM. From: Kuric E, Ruscher K. Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain--influence of levodopa treatment..)
Immunohistochemistry (IHC)
(MBS607599 used for the identification of activated microglia in rat brain by immunohistochemistry on formalin fixed free-floating sections. Spatiotemporal expression of MHC II protein in the ischemic hemisphere. Spatiotemporal distribution of MHC II expressing cells in the ipsilateral cortex of a sham operated animal and at the indicated time points after tMCAO. The border of the infarct core is delineated by a dotted line. Scale bars: low magnification - 200um; higher magnification - 20um. Abbreviations: V, lateral ventricle; IC, infarct core.)
Immunohistochemistry (IHC)
(MBS607599 used for the identification of activated microglia in rat brain by immunohistochemistry on formalin fixed free-floating sections. Spatiotemporal expression of MHC II protein in the ischemic hemisphere. Spatiotemporal distribution of MHC II expressing cells in the ipsilateral cortex of a sham operated animal and at the indicated time points after tMCAO. The border of the infarct core is delineated by a dotted line. Scale bars: low magnification - 200um; higher magnification - 20um. Abbreviations: V, lateral ventricle; IC, infarct core.)
Testing Data
(Staining of rat spleen cells with MBS607599.)
Testing Data
(Staining of rat spleen cells with MBS607599.)
Testing Data
(A488 conjugated mouse anti rat CD45RA and A647 conjugated mouse IgG1 isotype control. Figure B. A488 conjugated mouse anti rat CD45RA and A647 conjugated mouse anti rat MHC-II. All experiments performed on red cell lysed rat blood gated on mononuclear cells.)
Testing Data
(A488 conjugated mouse anti rat CD45RA and A647 conjugated mouse IgG1 isotype control. Figure B. A488 conjugated mouse anti rat CD45RA and A647 conjugated mouse anti rat MHC-II. All experiments performed on red cell lysed rat blood gated on mononuclear cells.)
Product Categories/Family for anti-MHC Class 2, I-Ak/s antibody
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Product Notes
The MHC Class 2, I-Ak/s (Catalog #AAA607599) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MHC Class 2, I-Ak/s (MHC Class II) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MHC Class 2, I-Ak/s can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Dilution: Flow Cytometry: 1:50-100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (paraffin): 1:50-1:100. Researchers should empirically determine the suitability of the MHC Class 2, I-Ak/s for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC Class 2, I-Ak/s, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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