Rabbit surfactant protein B ELISA Kit | SFTPB elisa kit

Rabbit Pulmonary surfactant-associated protein B, SFTPB ELISA Kit

Cat. Num. AAA941584

• Gene Names: SFTPB; SP-B; SFTP3
• Reactivity: Rabbit
• Synonyms: surfactant protein B; Rabbit Pulmonary surfactant-associated protein B; SFTPB ELISA Kit; Rabbit Pulmonary surfactant-associated protein B (SFTPB) ELISA kit; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253; Pulmonary surfactant-associated protein B; 18kD; surfactant; pulmonary-associated protein B; SFTPB elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rabbit SFTPB. No significant cross-reactivity or interference between Rabbit SFTPB and analogues was observed.
• Sequence Length: 369

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 1.87 ng/ml-120 ng/ml
• Sensitivity: 0.46 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Transport
• Protein Biological Process 3: Gaseous exchange
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFTPB elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFTPB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFTPB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPB bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126723505
• NCBI GeneID: 100009194
• NCBI Accession #: NP_001075812.1
• NCBI GenBank Nucleotide #: NM_001082343.1
• UniProt Accession #: P15285; P79333
• Molecular Weight: 40,610 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein B
• NCBI Official Symbol: SFTPB
• NCBI Official Synonym Symbols: SP-B; SFTP3
• NCBI Protein Information: pulmonary surfactant-associated protein B; 6 kDa protein; pulmonary surfactant-associated proteolipid SPL(Phe)
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPB
• UniProt Synonym Gene Names: SFTP3; SP-B
• UniProt Entry Name: PSPB_RABIT

Uniprot Description

Function: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.