Human Vacuolar protein sorting associated protein 53 homolog (VPS53) ELISA Kit | VPS53 elisa kit

Human Vacuolar protein sorting associated protein 53 homolog (VPS53) ELISA Kit

Cat. Num. AAA7200893

• Gene Names: VPS53; HCCS1; PCH2E; hVps53L; pp13624
• Reactivity: Human
• Synonyms: Vacuolar protein sorting associated protein 53 homolog (VPS53); Human Vacuolar protein sorting associated protein 53 homolog (VPS53) ELISA Kit; VPS53 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This VPS53 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VPS53. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VPS53 elisa kit

Principle of the assay: VPS53 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VPS53 antibody and an VPS53-HRP conjugate. The assay sample and buffer are incubated together with VPS53-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VPS53 concentration since VPS53 from samples and VPS53-HRP conjugate compete for the anti-VPS53 antibody binding site. Since the number of sites is limited, as more sites are occupied by VPS53 from the sample, fewer sites are left to bind VPS53-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VPS53 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for VPS53 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 21361728
• NCBI GeneID: 55275
• NCBI Accession #: NP_060759.2
• NCBI GenBank Nucleotide #: NM_018289.3
• Molecular Weight: 94,405 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 53 homolog isoform 2
• NCBI Official Synonym Full Names: vacuolar protein sorting 53 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS53
• NCBI Official Synonym Symbols: HCCS1; PCH2E; hVps53L; pp13624
• NCBI Protein Information: vacuolar protein sorting-associated protein 53 homolog
• UniProt Protein Name: Vacuolar protein sorting-associated protein 53 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS53
• UniProt Synonym Gene Names: PP13624
• UniProt Entry Name: VPS53_HUMAN

NCBI Description

This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS53: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Belongs to the VPS53 family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; endosome membrane

Molecular Function: protein binding

Biological Process: protein transport; retrograde transport, endosome to Golgi

Disease: Pontocerebellar Hypoplasia, Type 2e