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Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power)

Rat MARCO Monoclonal Antibody | anti-MARCO antibody

RAT ANTI MOUSE MARCO

Gene Names
Marco; Ly112; Scara2; AI323439
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Synonyms
MARCO; Monoclonal Antibody; RAT ANTI MOUSE MARCO; anti-MARCO antibody
Ordering
For Research Use Only!
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ED31
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
518
Applicable Applications for anti-MARCO antibody
Immunohistology Frozen, Immunofluorescence (IF), Western Blot (WB)
Application Notes
Immunohistology - Frozen: Minimum Dilution: 1/200; Maximum Dilution: 1/400
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
CHO cells expressing mouse MARCO
Fusion Partners
Spleen cells from immunised rats were fused with cells of the mouse SP2/0 myeloma cell line.
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
RRID
AB_2140591
Carrier Free
Yes
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power)

Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power)

Testing Data

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image wiith nuclei counterstained blue using DAPI. high power)

Testing Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image wiith nuclei counterstained blue using DAPI. high power)

Testing Data

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image wiith nuclei counterstained blue using DAPI. Low power)

Testing Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image wiith nuclei counterstained blue using DAPI. Low power)

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power)

Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power)

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse MARCO: FITC (MBS210787))

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse MARCO: FITC (MBS210787))

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)

Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 (MBS215278) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)
Related Product Information for anti-MARCO antibody
Rat anti Mouse MARCO antibody, clone ED31 recognizes the murine cell surface antigen designated MARCO (Macrophage receptor with collagenous structure), which is a member of the class A scavenger receptor family.

MARCO is expressed by distinct populations of macrophages in the spleen and lymph nodes, but is rapidly induced on macrophages in other tissues (e.g. Liver Kupffer cells) during infection or LPS treatment.

Rat anti Mouse MARCO antibody, clone ED31 binds to the C-terminal cysteine rich domain of MARCO, and has been shown to block ligand binding.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
macrophage receptor MARCO
NCBI Official Synonym Full Names
macrophage receptor with collagenous structure
NCBI Official Symbol
Marco
NCBI Official Synonym Symbols
Ly112; Scara2; AI323439
NCBI Protein Information
macrophage receptor MARCO
UniProt Protein Name
Macrophage receptor MARCO
Protein Family
UniProt Gene Name
Marco
UniProt Entry Name
MARCO_MOUSE

Uniprot Description

MARCO: Pattern recognition receptor (PRR). Binds Gram-positive and Gram-negative bacteria.

Protein type: Membrane protein, integral

Cellular Component: collagen; membrane; integral to membrane

Molecular Function: scavenger receptor activity

Biological Process: immune system process; innate immune response; endocytosis; apoptotic cell clearance

Research Articles on MARCO

Similar Products

Product Notes

The MARCO marco (Catalog #AAA215282) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MARCO can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Immunofluorescence (IF), Western Blot (WB). Immunohistology - Frozen: Minimum Dilution: 1/200; Maximum Dilution: 1/400. Researchers should empirically determine the suitability of the MARCO marco for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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