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Testing Data (Immunoperoxidase staining of a murine lymph node cryosections with Rat anti Mouse CD19 antibody, clone 6D5 followed by horseradish peroxidase conjugated Goat anti Rat IgG. Low power)

Rat anti-Mouse CD19 Monoclonal Antibody | anti-CD19 antibody

Rat anti Mouse CD19:Amethyst Orange

Gene Names
Cd19; AW495831
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CD19; Monoclonal Antibody; Rat anti Mouse CD19:Amethyst Orange; CD19 antibody | 6D5; anti-CD19 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6D5
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Amethyst Orange - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD19 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: Neat to 1/10
Immunogen
Human K562 cell line transfected with murine CD19.
Fusion Partners
Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
Conjugation
Amethyst Orange
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Testing Data

(Immunoperoxidase staining of a murine lymph node cryosections with Rat anti Mouse CD19 antibody, clone 6D5 followed by horseradish peroxidase conjugated Goat anti Rat IgG. Low power)

Testing Data (Immunoperoxidase staining of a murine lymph node cryosections with Rat anti Mouse CD19 antibody, clone 6D5 followed by horseradish peroxidase conjugated Goat anti Rat IgG. Low power)

Testing Data

(Immunoperoxidase staining of a murine lymph node cryosections with Rat anti Mouse CD19 antibody, clone 6D5 followed by horseradish peroxidase conjugated Goat anti Rat IgG. High power)

Testing Data (Immunoperoxidase staining of a murine lymph node cryosections with Rat anti Mouse CD19 antibody, clone 6D5 followed by horseradish peroxidase conjugated Goat anti Rat IgG. High power)

Immunofluorescence (IF)

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD19 antibody, clone 6D5 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using )

Immunofluorescence (IF) (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD19 antibody, clone 6D5 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using )

Immunofluorescence (IF)

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 , green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using )

Immunofluorescence (IF) (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 , green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using )

Testing Data

(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD19 . All experiments performed on )

Testing Data (Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD19 . All experiments performed on )

Testing Data

(Figure A. RPE conjugated rat anti mouse CD3 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated rat anti mouse CD3 and Alexa647 conjugated rat anti mouse CD19 . All experiments perf)

Testing Data (Figure A. RPE conjugated rat anti mouse CD3 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated rat anti mouse CD3 and Alexa647 conjugated rat anti mouse CD19 . All experiments perf)

Testing Data

(Figure A. FITC conjugated rat anti mouse CD16/CD32 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. FITC conjugated rat anti mouse CD16/CD32 and Alexa647 conjugated rat anti mouse CD19 . All exp)

Testing Data (Figure A. FITC conjugated rat anti mouse CD16/CD32 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. FITC conjugated rat anti mouse CD16/CD32 and Alexa647 conjugated rat anti mouse CD19 . All exp)

Testing Data

(Figure A. FITC conjugated Rat anti Mouse CD19 . Figure B. FITC conjugated Rat anti Mouse CD19 and SBV515 conjugated Rat anti Mouse CD45R . All experiments performed on red cell lysed mouse blood gated on live single cel)

Testing Data (Figure A. FITC conjugated Rat anti Mouse CD19 . Figure B. FITC conjugated Rat anti Mouse CD19 and SBV515 conjugated Rat anti Mouse CD45R . All experiments performed on red cell lysed mouse blood gated on live single cel)

Testing Data

(Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD45R . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD45R and SBV515 conjugated Rat anti Mouse CD19 . All experiments performed on red cell lysed mouse b)

Testing Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD45R . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD45R and SBV515 conjugated Rat anti Mouse CD19 . All experiments performed on red cell lysed mouse b)

Testing Data

(Figure A. FITC conjugated Rat anti Mouse CD19 . Figure B. FITC conjugated Rat anti Mouse CD19 and SBV440 conjugated Rat anti Mouse CD45R . All experiments performed on mouse splenocytes in the presence of 10% mouse seru)

Testing Data (Figure A. FITC conjugated Rat anti Mouse CD19 . Figure B. FITC conjugated Rat anti Mouse CD19 and SBV440 conjugated Rat anti Mouse CD45R . All experiments performed on mouse splenocytes in the presence of 10% mouse seru)
Related Product Information for anti-CD19 antibody
Rat anti Mouse CD19 antibody, clone 6D5 recognizes the murine CD19 cell surface antigen, a ~95kDa glycoprotein expressed by B lymphocytes. Rat anti Mouse CD19 antibody, clone 6D5 recognizes the same, or a closely related epitope as clone Rat anti Mouse CD19 antibody, clone ID3 in cross-competition assays.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,149 Da
NCBI Official Full Name
B-lymphocyte antigen CD19
NCBI Official Synonym Full Names
CD19 antigen
NCBI Official Symbol
Cd19
NCBI Official Synonym Symbols
AW495831
NCBI Protein Information
B-lymphocyte antigen CD19; differentiation antigen CD19
UniProt Protein Name
B-lymphocyte antigen CD19
Protein Family
UniProt Gene Name
Cd19
UniProt Entry Name
CD19_MOUSE

Research Articles on CD19

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Product Notes

The CD19 cd19 (Catalog #AAA226107) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat anti Mouse CD19:Amethyst Orange reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD19 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Neat to 1/10. Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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