Rat LGR5 (GPR49) Monoclonal Antibody | anti-LGR5 antibody

Rat anti-human LGR5 (GPR49) monoclonal antibody

Cat. Num. AAA468050

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Applications: Flow Cytometry, Functional Assay, ELISA
• Purity: Protein G
• Synonyms: LGR5 (GPR49); Monoclonal Antibody; Rat anti-human LGR5 (GPR49) monoclonal antibody; anti-LGR5 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a/kappa
• Clone Number: BEE-6F7
• Specificity: anti-human LGR5 (GPR49)
• Purity/Purification: Protein G
• Sequence Length: 835

• Applicable Applications for anti-LGR5 antibody: Flow Cytometry (FC/FACS), C-ELISA (EIA)
• Application Notes: Flow Cytometry: 1.2 mug/10^6 cells; C-ELISA: 1:200 - 1:400
• Buffer: Phosphate buffered saline, pH 7.2
• Immunogen: Genetic immunisation with cDNA encoding human LGR5
• Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
• Preparation and Storage: Short term: 2 degree C - 8 degree C; Long term: -20 degree C (avoid repeated freezing and thawing)

Flow Cytometry (FC/FACS)

(Fig.1: FACS analysis of BOSC23 cells using BEE-6F7. BOSC23 cells were transiently transfected with an expression vector encoding either LGR5-hum (green curve), or an irrelevant protein (control transfectant (red curve). Binding of BEE-6F7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with LGR5-hum transfected cells.)

Testing Data

(Fig.2: CGE analysis of purified GM-0607 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified GM-0607 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)

Related Product Information for anti-LGR5 antibody

LGR5 (Leucine-rich repeat- containing G protein-coupled receptor 5), also known as GPR49. belongs to the superfamily of G protein-coupled receptors (GPCRs) (1). It is a 907 amino acid multi-pass membrane protein and has multiple N-terminal leucine-rich repeats, which are important for interaction with the glycoprotein ligands, and 7 transmembrane domains. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues (2). LGR5 is a member of the Wnt signaling pathway. Although its ligand remains elusive, it has been shown that costimulation with R-spondin 1 and Wnt-3a induce increased internalization of LGR5 (3). LGR5 is overexpressed in certain cancer types, e.g. gastrointestinal tumours and is thought to represent a tumour suppressor, although its role there is still unclear.

References

1. McDonald T, Wang R, Bailey W, Xie G, Chen F, Caskey CT, Liu Q (1998). Identification and cloning of an orphan G protein-coupled receptor of the glycoprotein hormone receptor subfamily". Biochem Biophys Res Commun 247 (2): 266-70
2. Hsu SY, Liang SG, Hsueh AJ (1998). Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G proteincoupled, seven-transmembrane region. Mol. Endocrinol. 12: 1830-45
3. de Lau W1, Barker N, Low TY, Koo BK, Li VS, Teunissen H, Kujala P, Haegebarth A, Peters PJ, van de Wetering M, Stange DE, van Es JE, Guardavaccaro D, Schasfoort RB, Mohri Y, Nishimori K, Mohammed S, Heck AJ, Clevers H.(2011) Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476: 293-7

NCBI and Uniprot Product Information

• NCBI GI #: 469608416
• NCBI GeneID: 8549
• NCBI Accession #: NP_001264156.1
• NCBI GenBank Nucleotide #: NM_001277227.1
• Molecular Weight: 92,006 Da
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5 isoform 3
• NCBI Official Synonym Full Names: leucine-rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67
• UniProt Entry Name: LGR5_HUMAN

NCBI Description

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

LGR5: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of cycling, long-lived and multipotent adult stem cell populations. Target gene of Wnt signaling. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Intestinal Lgr5+ stem cells give rise throughout life to proliferation of cells in crypts, which migrate along the crypt-villus axis, differentiating into five main cell types. Wnt signaling plays a crucial role in the proliferation and differentiation of these crypt epithelial cells. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: integral to plasma membrane; plasma membrane; trans-Golgi network membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; hair follicle development; inner ear development; oocyte differentiation; regulation of cell proliferation