Rabbit GPR49 / LGR5 Polyclonal Antibody | anti-GPR49 antibody

Rabbit Polyclonal to Human GPR49 / LGR5

Cat. Num. AAA248718

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Reactivity: Bat, Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: GPR49 / LGR5; Polyclonal Antibody; Rabbit Polyclonal to Human GPR49 / LGR5; Anti-GPR49 / LGR5 Antibody (N-Terminus) IHC-plus; LGR5; FEX; G-protein coupled receptor 49; G protein-coupled receptor 49; GPR67; GRP49; GPR49; HG38; G-protein coupled receptor 67; Human GPR49; anti-GPR49 antibody

• Host: Rabbit
• Reactivity: Bat, Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GPR49 / LGR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 907

• Applicable Applications for anti-GPR49 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (1:400)
• Target Species: Human
• Immunogen Description: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human GPR49 / LGR5. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%); Elephant, Panda (95%); Mouse, Rat, Bovine (90%).
• Immunogen Type: Synthetic peptide
• Immunogen: GPR49 / LGR5 antibody was raised against synthetic 20 amino acid peptide from N-terminal extracellular domain of human GPR49 / LGR5. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%); Elephant, Panda (95%); Mouse, Rat, Bovine (90%); Opossum (85%); Rabbit, Chicken (80%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Human, Pancreas, Islets: Formalin-Fixed Paraffin-Embedded (FFPE))

Related Product Information for anti-GPR49 antibody

GPR49 is an Orphan-A GPCR with an unknown ligand.

Product Categories/Family for anti-GPR49 antibody

Family: GPCR; Subfamily: Orphan-A

NCBI and Uniprot Product Information

• NCBI GI #: 4504379
• NCBI GeneID: 8549
• NCBI Accession #: NP_003658.1
• NCBI GenBank Nucleotide #: NM_003667.3
• UniProt Accession #: O75473; Q4VAM0; Q4VAM2; Q9UP75; D8MCT0
• Molecular Weight: 92,006 Da
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5 isoform 1
• NCBI Official Synonym Full Names: leucine-rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; orphan G protein-coupled receptor HG38; leucine-rich repeat-containing G protein-coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67
• UniProt Entry Name: LGR5_HUMAN

NCBI Description

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

LGR5: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of cycling, long-lived and multipotent adult stem cell populations. Target gene of Wnt signaling. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Intestinal Lgr5+ stem cells give rise throughout life to proliferation of cells in crypts, which migrate along the crypt-villus axis, differentiating into five main cell types. Wnt signaling plays a crucial role in the proliferation and differentiation of these crypt epithelial cells. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: integral to plasma membrane; plasma membrane; trans-Golgi network membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; inner ear development