Rabbit LGR5 Polyclonal Antibody | anti-LGR5 antibody

LGR5 antibody

Cat. Num. AAA5309891

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Applications: Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: LGR5; Polyclonal Antibody; LGR5 antibody; Rabbit polyclonal LGR5 antibody; Polyclonal LGR5 antibody; Anti-LGR5 antibody; LGR 5 antibody; LGR-5; LGR 5; Leucine-rich repeat-containing G-protein coupled receptor 5 antibody; LGR-5 antibody; GPR49 antibody; FEX antibody; anti-LGR5 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Polyclonal LGR5 antibody specific for Human LGR5.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 907

• Applicable Applications for anti-LGR5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: LGR5 antibody was raised in Rabbit using the peptide sequence around aa 490~494(K-I-S-N-Q) derived from Human LGR5(GPR49) as the immunogen.
• Cross-Reactivity: Human, Mouse
• Post-Translational Modifications: LGR5 undergoes glycosylation2 at Asn63, Asn77, Asn208, Asn500 and Asn792.
• Preparation and Storage: Store at 4 degree C for short term storage.; Aliquot and store at-20 degree C for long term storage.; Avoid repeated freeze/thaw cycles.

Testing Data

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Western Blot (WB)

(Western Blot analysis of extracts from Hela, 3T3 and HEPG2 cells using LGR5 antibody (20R-2773))

Related Product Information for anti-LGR5 antibody

Biological Significance: LGR5 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), LGR5 associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Sequence Info: LGR5 is composed of 907 amino acids with a molecular weight of 99998 Da. There is a Leucine-rich repeat-containing G-protein coupled receptor 5 chain at amino acids 22 907 and a signal peptide at positions 1 21.
Implications in Disease: LGR5 stem cells were identified to fuel stem cell activity in murine intestinal adenomas via erroneous activation of the pro-cell cycle Wnt signalling pathway as a result of successive mutations, such as formation of adenoma via Adenomatous polyposis coli (APC) mutation. Loss of LGR5 actually increased tumourigenicity and invasion whereas overexpression results a reduction in tumourigenicity and clonogenicity. This implies that LGR5 is not an oncogene but a tumor suppressor gene, and that its main role is delimiting stem cell expansion in their respective niches. Varying expression profile of LGR5 was also observed in different stages of gastrointestinal cancers, which suggests that the histoanatomical distribution of LGR5+ve stem cells determine how the cancer advances.
Expression Note: Highly expressed in neural cells and is overexpressed in cancers of the ovary, colon and liver. In premalignant cancer expression is not restricted to the cript base. LGR5 is an integral membrane protein expressed in skeletal muscle, placenta, spinal cord, and various region of brain. LGR5 is also expressed at the base of crypts in colonic and small mucosa stem cells.

Product Categories/Family for anti-LGR5 antibody

Differentiation & Development

NCBI and Uniprot Product Information

• NCBI GI #: 21542118
• NCBI GeneID: 8549
• UniProt Accession #: O75473; Q4VAM0; Q4VAM2; Q9UP75; D8MCT0
• Molecular Weight: 92,006 Da
• NCBI Official Full Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• NCBI Official Synonym Full Names: leucine rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67

NCBI Description

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.