Rabbit anti-Human, mouse Leucine-rich repeat-containing G-protein coupled receptor 5, LGR5 Polyclonal Antibody | anti-LGR5 antibody

Anti-Leucine-rich repeat-containing G-protein coupled receptor 5, LGR5 antibody

Cat. Num. AAA355156

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Reactivity: Human, mouse
• Applications: Western Blot, Immunocytochemistry
• Purity: Immunogen affinity purified
• Synonyms: Leucine-rich repeat-containing G-protein coupled receptor 5; LGR5; Polyclonal Antibody; Anti-Leucine-rich repeat-containing G-protein coupled receptor 5; LGR5 antibody; Polyclonal Anti- Leucine-rich repeat-containing G-protein coupled receptor 5; anti-LGR5 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid

• Applicable Applications for anti-LGR5 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB (1:1000-2000); IHC-P (1:200); ICC (1:50)
• Immunogen: A synthetic peptide (conjugated to KLH) corresponding to the region of LGR5.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Related Product Information for anti-LGR5 antibody

Leucine-rich repeat-containing G-protein coupled receptor 5(LGR5), also known as GPR49, is a 907-amino acid 7-transmembrane protein, [1] belongs to G Protein-Coupled Receptor. The differential expression of GPR49 between normal and tumor tissue was significant. GPR49 was upregulated in colon primary tumor tissues. In addition to colon tumors, GPR49 was also found to be upregulated in ovarian primary tumor tissues. Moreover, the expression level of GPR49 in colon and ovarian tumors increased in more advanced tumors suggesting a role for the receptor in tumor progression. [2]

References

1. Hsu, S. Y., Liang, S.-G., Hsueh, A. J. W. Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven-transmembrane region. Molec. Endocr. 12: 1830-1845, 1998. 2. McClanahan T, Koseoglu S, Smith K et al. (2007). "Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors". Cancer Biol. Ther. 5 (4): 419-26.

NCBI and Uniprot Product Information

• NCBI GI #: 4504379
• NCBI GeneID: 8549
• NCBI Accession #: NP_003658.1
• NCBI GenBank Nucleotide #: NM_003667.3
• UniProt Accession #: O75473; Q4VAM0; Q4VAM2; Q9UP75
• Molecular Weight: 99,998 Da
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5 isoform 1
• NCBI Official Synonym Full Names: leucine-rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; orphan G protein-coupled receptor HG38; leucine-rich repeat-containing G protein-coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67
• UniProt Entry Name: LGR5_HUMAN

NCBI Description

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location: Cell membrane; Multi-pass membrane protein. Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein. Note: Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans-Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (Ref.12). Ref.10 Ref.11 Ref.12

Tissue specificity: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. Ref.5 Ref.6 Ref.7

Miscellaneous: LGR5 is used as a marker of adult tissue stem cells in the intestine, stomach, hair follicle, and mammary epithelium (Ref.7).

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.Contains 16 LRR (leucine-rich) repeats.Contains 1 LRRNT domain.