Mouse Lamin A,C Monoclonal Antibody | anti-lmna antibody

Lamin A,C

Cat. Num. AAA606287

• Gene Names: lmna; lmna-A
• Reactivity: Bovine, Canine, Hamster, Human, Mouse, Rat, Sheep
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Lamin A; C; Monoclonal Antibody; Anti -Lamin A; anti-lmna antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Hamster, Human, Mouse, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4A59
• Specificity: Reacts against lamins A and C. Epitope located between residues 319-566. Species Crossreactivity: Human, mouse, rat, bovine, hamster, canine, and sheep.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.

• Applicable Applications for anti-lmna antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Immunohistochemistry (frozen sections), Flow Cytometry, and Western Blot.; Dilution: Western Blot: 1:50-1:500; Immunohistochemistry: 1:50-1:100; Flow Cytometry: 1:50-1:100; Optimum dilutions to be determined by researcher.
• Immunogen: Lamin A,C
• Hybridoma: P3X63-Ag8.653myeloma cells w/spleen cells from Balb/c mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-lmna antibody

An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of a-helices and a carboxyterminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organised into loops, which are attached to the nuclear matrix. This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins.

Product Categories/Family for anti-lmna antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 156119433
• NCBI GeneID: 373673
• NCBI Accession #: NP_001095210.1
• NCBI GenBank Nucleotide #: NM_001101740.1
• UniProt Accession #: P11048; AAB23219
• Molecular Weight: 74,919 Da
• NCBI Official Full Name: lamin-A
• NCBI Official Synonym Full Names: lamin A/C
• NCBI Official Symbol: lmna
• NCBI Official Synonym Symbols: lmna-A
• NCBI Protein Information: lamin-A
• UniProt Protein Name: Lamin-A
• Protein Family: Lamin
• UniProt Gene Name: lmna
• UniProt Entry Name: LMNA_XENLA

Uniprot Description

Function: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Subcellular location: Nucleus.

Miscellaneous: There are at least five different lamins in Xenopus: the somatic lamins L(I), L(II), and A; the oocyte germinal vesicle lamin L(III); and the male germ cells lamin l(IV).

Sequence similarities: Belongs to the intermediate filament family.