Mouse Lamin B2 Monoclonal Antibody | anti-LMNB2 antibody

Mouse anti Lamin B2

Cat. Num. AAA570089

• Gene Names: LMNB2; LMN2; LAMB2; MGC2721
• Reactivity: Hamster, Human, Mouse, Swine, Xenopus, Zebrafish
• Applications: Flow Cytometry, Immunohistochemistry, Western Blot
• Synonyms: Lamin B2; Monoclonal Antibody; Mouse anti Lamin B2; anti-LMNB2 antibody

• Host: Mouse
• Reactivity: Hamster, Human, Mouse, Swine, Xenopus, Zebrafish
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: LN43
• Specificity: LN43 reacts with an epitope loCated in the C-terminal part of lamin B2.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-LMNB2 antibody: Flow Cytometry (FC), Immunohistochemistry (IHC) (frozen), Western Blot (WB)
• Application Notes: LN43 is suitable for immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
• Source Note: LN43 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with the detergent insoluble fraction of potoroo cell line PtK1.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Immunohistochemistry (IHC)

(Figure 1. immunohistochemistry on frozen sections of human kidney showing nuclear lamina staining in the ductal epithelium.)

Immunofluorescence (IF)

(Figure 2. immunofluorescence staining of a 9 days old zebrafish embryo.)

Immunohistochemistry (IHC)

(Figure 3. immunohistochemistry on a frozen section of human kidney showing nuclear lamina staining in the ductal epithelium.)

Related Product Information for anti-LMNB2 antibody

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, ie lamin B1 and lamin B2.

Product Categories/Family for anti-LMNB2 antibody

Nuclear proteins; Cytoskeleton

References

Bridger, J. M., Kill, I. R., O'Farrell, M., and Hutchison, C. J. (1993). Internal lamin structures within G1 nuclei of Human dermal fibroblasts, J Cell Sci 104, 297-306. Jenkins, H., Hölman, T., Lyon, C., Lane, B., Stick, R., and Hutchison, C. J. (1993). Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix, J Cell Sci 106, 275-285. Hozak, P., Sasseville, A. M., Raymond, Y., and Cook, P. R. (1995). Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in Human cells, J Cell Sci 108, 635-44. Machiels, B. M., Broers, J. L., Raymond, Y., de Ley, L., Kuijpers, H. J., Caberg, N. E., and Ramaekers, F. C. (1995). Abnormal A-type lamin organization in a Human lung carcinoma cell line, Eur J Cell Biol 67, 328-35. Machiels, B. M., Zorenc, A. H., Endert, J. M., Kuijpers, H. J., van Eys, G. J., Ramaekers, F. C., and Broers, J. L. (1996). An alternative splicing product of the lamin A/C gene lacks exon 10, J Biol Chem 271, 9249-53. Machiels, B. M., Ramaekers, F. C., Kuijpers, H. J., Groenewoud, J. S., Oosterhuis, J. W., and Looijenga, L. H. (1997). Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults, J Pathol 182, 197-204. Jansen, M. P., Machiels, B. M., Hopman, A. H., Broers, J. L., Bot, F. J., Arends, J. W., Ramaekers, F. C., and Schouten, H. C. (1997). Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease, Histopathology 31, 304-12. Broers, J. L., Machiels, B. M., Kuijpers, H. J., Smedts, F., van den Kieboom, R., Raymond, Y., and Ramaekers, F. C. (1997). A- and B-type lamins are differentially expressed in normal Human tissues, Histochem Cell Biol 107, 505-17.

NCBI and Uniprot Product Information

• NCBI GI #: 1054874
• NCBI GeneID: 84823
• UniProt Accession #: Q03252; O75292; Q14734; Q96DF6
• Molecular Weight: 67,689 Da
• NCBI Official Full Name: lamin B2
• NCBI Official Synonym Full Names: lamin B2
• NCBI Official Symbol: LMNB2
• NCBI Official Synonym Symbols: LMN2; LAMB2; MGC2721
• NCBI Protein Information: lamin-B2; OTTHUMP00000198023
• UniProt Protein Name: Lamin-B2
• Protein Family: Lamin
• UniProt Gene Name: LMNB2
• UniProt Synonym Gene Names: LMN2
• UniProt Entry Name: LMNB2_HUMAN

NCBI Description

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq]

Uniprot Description

Lamin B2: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Lamin B2 may be lost during the follicle centre stage of B-cell differentiation in humans. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: lamin filament; nuclear inner membrane

Molecular Function: structural molecule activity

Disease: Lipodystrophy, Partial, Acquired, Susceptibility To