Mouse Lamin B1 Monoclonal Antibody | anti-LMNB1 antibody

Mouse anti Lamin B1

Cat. Num. AAA570056

• Gene Names: LMNB1; LMN; ADLD; LMN2; LMNB; MGC111419
• Reactivity: Bovine, Canine, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: Lamin B1; Monoclonal Antibody; Mouse anti Lamin B1; anti-LMNB1 antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 119D5-F1
• Specificity: 119D5-F1 reacts with an epitope loCated C-terminal of residue 231 in lamin B1.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-LMNB1 antibody: Flow Cytometry (FC), Immunocytochemistry (IHC), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: 119D5-F1 is suitable for immunocytochemistry on permeabilised cells, immunohistochemistry on frozen tissues, immunoblotting, ELISA and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, immunocytochemistry and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
• Source Note: 119D5-F1 is a Mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with purified Rat liver lamins.
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Immunohistochemistry (IHC)

(Figure 1. Immunohistochemistry of MCF-7 cell culture showing nuclear lamina staining.)

Immunohistochemistry (IHC)

(Figure 2. Immunohistochemistry on frozen sections of swine liver showing nuclear lamina staining in hepatocytes.)

Immunohistochemistry (IHC)

(Figure 3. Immunohistochemistry on frozen sections of human kidney showing nuclear lamina staining in epithelial and connective tissue cells.)

Related Product Information for anti-LMNB1 antibody

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Lamins play a crucial role in the maintenence of nuclear structure, gene regulation and signalling from cytoplasm to nucleus. Mutations in lamins have been associated with a number of pathologies; the so-called laminopathies.

Product Categories/Family for anti-LMNB1 antibody

Nuclear proteins; Cytoskeleton

References

Weaver, V. M., Carson, C. E., Walker, P. R., Chaly, N., Lach, B., Raymond, Y., Brown, D. L., and Sikorska, M. (1996). Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes, J Cell Sci 109, 45-56. Pugh, G. E., Coates, P. J., Lane, E. B., Raymond, Y., and Quinlan, R. A. (1997). Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells, J Cell Sci 110, 2483-93. Broers, J. L., Machiels, B. M., Kuijpers, H. J., Smedts, F., van den Kieboom, R., Raymond, Y., and Ramaekers, F. C. (1997). A- and B-type lamins are differentially expressed in normal Human tissues, Histochem Cell Biol 107, 505-17. Machiels, B. M., Broers, J. L., Raymond, Y., de Ley, L., Kuijpers, H. J., Caberg, N. E., and Ramaekers, F. C. (1995). Abnormal A-type lamin organization in a Human lung carcinoma cell line, Eur J Cell Biol 67, 328-35. Machiels, B. M., Ramaekers, F. C., Kuijpers, H. J., Groenewoud, J. S., Oosterhuis, J. W., and Looijenga, L. H. (1997). Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults, J Pathol 182, 197-204. Jansen, M. P., Machiels, B. M., Hopman, A. H., Broers, J. L., Bot, F. J., Arends, J. W., Ramaekers, F. C., and Schouten, H. C. (1997). Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease, Histopathology 31, 304-12. Neri, L. M., Raymond, Y., Giordano, A., Capitani, S., and Martelli, A. M. (1999). Lamin A is part of the internal nucleoskeleton of Human erythroleukemia cells, J Cell Physiol 178, 284-95. Broers, J. L., Bronnenberg, N. M., Kuijpers, H. J., Schutte, B., Hutchison, C. J., and Ramaekers, F. C. (2002). Partial cleavage of A-type lamins concurs with their total disintegRation from the nuclear lamina during apoptosis. Eur J Cell Biol 81, 677-691.

NCBI and Uniprot Product Information

• NCBI GI #: 576840
• NCBI GeneID: 4001
• UniProt Accession #: P20700; Q3SYN7; Q96EI6; B2R6J6; Q6DC98
• NCBI Official Full Name: lamin B1
• NCBI Official Synonym Full Names: lamin B1
• NCBI Official Symbol: LMNB1
• NCBI Official Synonym Symbols: LMN; ADLD; LMN2; LMNB; MGC111419
• NCBI Protein Information: lamin-B1; OTTHUMP00000159218
• UniProt Protein Name: Lamin-B1
• Protein Family: Lamin
• UniProt Gene Name: LMNB1
• UniProt Synonym Gene Names: LMN2; LMNB
• UniProt Entry Name: LMNB1_HUMAN

NCBI Description

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq]

Uniprot Description

Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope

Molecular Function: phospholipase binding; structural molecule activity

Biological Process: apoptosis; cell structure disassembly during apoptosis

Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant