Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vasodilator-stimulated phosphoprotein (VASP) Standard Curve.)
Human Vasodilator-Stimulated Phosphoprotein (VASP) ELISA Kit | VASP elisa kit
Human Vasodilator-Stimulated Phosphoprotein (VASP) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vasodilator-stimulated phosphoprotein (VASP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vasodilator-stimulated phosphoprotein (VASP) Standard Curve.)
Background: Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. Northern blot analysis revealed that a 5.8-kb transcript is expressed in electromotor neurons. Western blot analysis determined expression of a 42-kD protein in the electric organ that copurified with synaptic vesicles.Smith et al. (1996) identified the sequence of 2 complete genes within 117 kb of DNA containing the BRCA1 gene: RHO7 and VAT1, an abundant membrane protein of cholinergic synaptic vesicles. The coding sequence of VAT1 predicts a 301-amino acid peptide. The authors found that a CpG island precedes the VAT1 gene, which contains 6 exons spanning 8.1 kb. They determined the following order of genes in this region: cen--IFP35--VAT1--RHO7--BRCA1--1A1-3B--tel.
NCBI and Uniprot Product Information
NCBI Description
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
Uniprot Description
VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: filopodium membrane; focal adhesion; tight junction; cytoplasm; plasma membrane; cytosol; actin cytoskeleton
Molecular Function: protein binding; actin binding; SH3 domain binding; profilin binding
Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization
Research Articles on VASP
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Product Notes
The Human VASP vasp (Catalog #AAA9713739) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713739 ELISA Kit recognizes Human VASP. It is sometimes possible for the material contained within the vial of "Vasodilator-Stimulated Phosphoprotein (VASP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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