Mouse anti-Human LACTB Monoclonal Antibody | anti-LACTB antibody

LACTB Mouse mAb

Cat. Num. AAA435164

• Gene Names: LACTB; G24; MRPL56
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: LACTB; Monoclonal Antibody; LACTB Mouse mAb; G24; mitochondrial; mitochondrial 39S ribosomal protein L56; mitochondrial ribosomal protein L56; MRPL56; serine beta lactamase-like protein; serine beta lactamase-like protein LACTB; serine beta-lactamase-like protein LACTB; Serine beta-lactamase-like protein LACTB; LACT-1; Lact1; UNQ843/PRO1781; anti-LACTB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 332

• Applicable Applications for anti-LACTB antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 10 tests; FC/FACS: 1:100-1:200; ; For Western Blot, incubate membrane with diluted antibody in 5% w/v nonfat dy milk, 1 x TBS, 0.05% Tween-20 at 4 degree C with gentle shaking overnight.
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human LACTB
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

Immunoprecipitation (IP)

(LACTB was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25101 of 10 ug. Western blot was performed from the immunopre-cipitate using M25101 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 61 KDaObserved band size : 61 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling LACTB with M25101 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)

Related Product Information for anti-LACTB antibody

This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms.

Product Categories/Family for anti-LACTB antibody

Cell Marker; Subcellular Marker; Membrane

NCBI and Uniprot Product Information

• NCBI GI #: 568384811
• NCBI GeneID: 114294
• NCBI Accession #: NP_001275514.1
• NCBI GenBank Nucleotide #: NM_001288585.1
• UniProt Accession #: P83111; P83096
• Molecular Weight: Predicted MW: 61kDa
• NCBI Official Full Name: serine beta-lactamase-like protein LACTB, mitochondrial isoform c
• NCBI Official Synonym Full Names: lactamase beta
• NCBI Official Symbol: LACTB
• NCBI Official Synonym Symbols: G24; MRPL56
• NCBI Protein Information: serine beta-lactamase-like protein LACTB, mitochondrial
• UniProt Protein Name: Serine beta-lactamase-like protein LACTB, mitochondrial
• Protein Family: Serine beta-lactamase-like protein
• UniProt Gene Name: LACTB

NCBI Description

This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Dec 2013]

Uniprot Description

Mitochondrial serine protease that acts as a regulator of mitochondrial lipid metabolism (PubMed:28329758). Acts by decreasing protein levels of PISD, a mitochondrial enzyme that converts phosphatidylserine (PtdSer) to phosphatidylethanolamine (PtdEtn), thereby affecting mitochondrial lipid metabolism (PubMed:28329758). It is unclear whether it acts directly by mediating proteolysis of PISD or by mediating proteolysis of another lipid metabolism protein (PubMed:28329758). Acts as a tumor suppressor that has the ability to inhibit proliferation of multiple types of breast cancer cells: probably by promoting decreased levels of PISD, thereby affecting mitochondrial lipid metabolism (PubMed:28329758).