Western Blot (WB)
Mouse anti-Human STRN3 Monoclonal Antibody | anti-STRN3 antibody
STRN3 (617) Mouse mAb
Gene Names
STRN3; SG2NA; S/G2NA; PPP2R6B
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
STRN3; Monoclonal Antibody; STRN3 (617) Mouse mAb; STRN3(617) Mouse mAb; Cell cycle autoantigen SG2NA; cell cycle S/G2 nuclear autoantigen; nuclear autoantigen; protein phosphatase 2 regulatory subunit B'''beta; S/G2 antigen; SG2NA; striatin; calmodulin binding protein 3; Striatin-3; GS2NA; PPP2R6B; anti-STRN3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Sequence Length
797
Applicable Applications for anti-STRN3 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200
IP: 10 tests
FC/FACS: 1:100-1:200
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human STRN3
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Western Blot (WB)
Western Blot (WB)
Immunoprecipitation (IP)
(STRN3 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25105 of 10 ug. Western blot was performed from the immunopre-cipitate using M25105 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 87 KDaObserved band size : 87 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Immunoprecipitation (IP)
(STRN3 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25105 of 10 ug. Western blot was performed from the immunopre-cipitate using M25105 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 87 KDaObserved band size : 87 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STRN3 with M25105 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STRN3 with M25105 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Related Product Information for anti-STRN3 antibody
Binds calmodulin in a calcium dependent manner. May function as scaffolding or signaling protein.
Product Categories/Family for anti-STRN3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 87kDa
NCBI Official Full Name
striatin-3 isoform 1
NCBI Official Synonym Full Names
striatin 3
NCBI Official Symbol
STRN3
NCBI Official Synonym Symbols
SG2NA; S/G2NA; PPP2R6B
NCBI Protein Information
striatin-3
UniProt Protein Name
Striatin-3
Protein Family
UniProt Gene Name
STRN3
UniProt Synonym Gene Names
GS2NA; SG2NA
Uniprot Description
Binds calmodulin in a calcium dependent manner. May function as scaffolding or signaling protein.CautionWas originally thought to be nuclear.
Research Articles on STRN3
Similar Products
Product Notes
The STRN3 strn3 (Catalog #AAA435160) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STRN3 (617) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STRN3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the STRN3 strn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STRN3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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