Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human tonsil stained with Ku Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells.)
Mouse Ku Monoclonal Antibody
Ku, p70, p80 (BSA & Azide Free) (MaxLight 490)
Reactivity
Human, Monkey, Mouse, Rat, Xenopus
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
Purified by Ammonium Sulfate Precipitation
Synonyms
Ku; Monoclonal Antibody; p70; p80 (BSA & Azide Free) (MaxLight 490); anti-Ku antibody
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Xenopus
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3F247
Specificity
Recognizes Ku, p70, p80. Species Crossreactivity: human, monkey, xenopus. mouse and rat (Immunoprecipitation only). Does not react with bovine and rabbit.
Purity/Purification
Purified by Ammonium Sulfate Precipitation
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-Ku antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Immunogen
Human B cell nuclei from plasmacytoid 2p68 cells
Conjugate
MaxLight490
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human tonsil stained with Ku Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human tonsil stained with Ku Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells.)
Product Categories/Family for anti-Ku antibody
References
1. Wang J, et. al. Molecular Biology Reports, 1993, 18(1):15-28. 2. Reeves WH, et. al. Journal of Experimental Medicine, 1985, 161(1):18-39. Additional: 1. Griffin W, Torrance H, Rodda DJ, Prefontaine GG, Pope L, Hache RJG. Sequence-specific DNA binding by autoantigen and its effects on transcription. Nature, 1996, 380:265-8. 2. Ajmani AK; Satoh M; Reap E; Cohen PL; Reeves WH. Absence of autoantigen Ku in mature human neutrophils and human promyelocytic leukemia line (HL-60) cells and lymphocytes undergoing apoptosis. Journal of Experimental Medicine, 1995 Jun 1, 181(6):2049-58. 3. Boubnov NV; Hall KT; Wills Z; Lee SE; He DM; Benjamin DM; Pulaski CR; Band H; Reeves W; Hendrickson EA; et al. Complementation of the ionizing radiation sensitivity, DNA end binding, and V(D)J recombination defects of double-strand break repair mutants by the p86 Ku autoantigen. Proceedings of the National Academy of Sciences of the United States of America, 1995 Jan 31, 92(3):890-4. 4. DiCroce PA; Krontiris TG. The BCL2 major breakpoint region is a sequence-and cell-cycle-specific binding site of the Ku antigen. Proceedings of the National Academy of Sciences of the United States of America, 1995 Oct 24, 92(22):10137-41. 5. Wang J; Chou CH; Blankson J; Satoh M; Knuth MW; Eisenberg RA; Pisetsky DS; Reeves WH. Murine monoclonal antibodies specific for conserved and nonconserved antigenic determinants of the human and murine Ku autoantigens. Molecular Biology Reports, 1993 Jun, 18(1):15-28. 6. Reeves WH. Use of monoclonal antibodies for the characterization of novel DNA-binding proteins recognized by human autoimmune sera. Journal of Experimental Medicine, 1985 Jan 1, 161(1):18-39.
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Product Notes
The Ku (Catalog #AAA6267470) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ku, p70, p80 (BSA & Azide Free) (MaxLight 490) reacts with Human, Monkey, Mouse, Rat, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Ku can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Ku for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ku, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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