Western Blot (WB)
((2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DPM3 Polyclonal Antibody | anti-DPM3 antibody
Goat anti-DPM3 Antibody
Western Blot: Approx 10+13kDa bands observed in lysates of cell line K562 (calculated MW of 13.3kDa according to NP_061846.2 and 10.1kDa according to NP_714963.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a subunit of dolichyl-phosphate mannosyltransferase and acts as a stabilizer subunit of the dolichyl-phosphate mannosyltransferase complex. [provided by RefSeq, Jul 2008]
Uniprot Description
DPM3: Stabilizer subunit of the dolichol-phosphate-mannose synthase complex. Defects in DPM3 are the cause of congenital disorder of glycosylation type 1O (CDG1O). A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the DPM3 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Transferase; Membrane protein, multi-pass; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; mannosyltransferase complex; membrane
Molecular Function: dolichyl-phosphate beta-D-mannosyltransferase activity; protein binding
Biological Process: carbohydrate metabolic process; GPI anchor biosynthetic process; protein amino acid C-linked glycosylation via 2'-alpha-mannosyl-L-tryptophan; protein amino acid mannosylation; protein amino acid N-linked glycosylation via asparagine; protein amino acid O-linked mannosylation; regulation of protein stability
Disease: Congenital Disorder Of Glycosylation, Type Io
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Product Notes
The DPM3 dpm3 (Catalog #AAA422934) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DPM3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's DPM3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 10+13kDa bands observed in lysates of cell line K562 (calculated MW of 13.3kDa according to NP_061846.2 and 10.1kDa according to NP_714963.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the DPM3 dpm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HDCEDAAREL QSQ. It is sometimes possible for the material contained within the vial of "DPM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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