Mouse Early Growth Response Protein 1 (EGR1) ELISA Kit | EGR1 elisa kit

Mouse Early Growth Response Protein 1 (EGR1) ELISA Kit

Cat. Num. AAA9718000

• Gene Names: Egr1; egr; TIS8; Zenk; Egr-1; NGFIA; Zfp-6; ETR103; Krox-1; Krox24; NGF1-A; NGFI-A; Zif268; Krox-24; A530045N19Rik
• Reactivity: Mouse
• Synonyms: Early Growth Response Protein 1 (EGR1); Mouse Early Growth Response Protein 1 (EGR1) ELISA Kit; EGR1; AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225; early growth response protein 1; nerve growth factor-induced protein A; transcription factor ETR103; zinc finger protein 225; EGR1 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Early growth response protein 1 (EGR1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse EGR1. No significant cross-reactivity or interference between Mouse EGR1 and analogues was observed.
• Sequence Length: 533

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Early growth response protein 1 (EGR1) Standard Curve.)

Related Product Information for EGR1 elisa kit

Description: This Mouse Early growth response protein 1 (EGR1) ELISA Kit employs a two-site sandwich ELISA to quantitate EGR1 in samples. An antibody specific for EGR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Zif268 is a mammalian transcription factor that is now officially known as Egr1.It was originally discovered in mouse. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Studies suggest this is a tumor suppressor gene.The DNA binding domain of Zif268 consists of three zinc finger domains of the Cys2His2 type. The amino acid structure of the Zif268 zinc finger domain is given in this table, using the single letter amino acid code. The fingers 1 to 3 are indicated by f1 - f3. The residues marked 'x' are not part of the zinc fingers, but rather serve to connect them all together.

NCBI and Uniprot Product Information

• NCBI GI #: 6681285
• NCBI GeneID: 13653
• NCBI Accession #: NP_031939.1
• NCBI GenBank Nucleotide #: NP_031939.1
• UniProt Accession #: P08046
• NCBI Official Full Name: early growth response protein 1
• NCBI Official Synonym Full Names: early growth response 1
• NCBI Official Symbol: Egr1
• NCBI Official Synonym Symbols: egr; TIS8; Zenk; Egr-1; NGFIA; Zfp-6; ETR103; Krox-1; Krox24; NGF1-A; NGFI-A; Zif268; Krox-24; A530045N19Rik
• NCBI Protein Information: early growth response protein 1
• UniProt Protein Name: Early growth response protein 1
• Protein Family: Early growth response protein
• UniProt Gene Name: Egr1
• UniProt Synonym Gene Names: NGFI-A

Uniprot Description

Transcriptional regulator (PubMed:8336701, PubMed:8703054, PubMed:15958557). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (PubMed:8703054, PubMed:15958557, PubMed:2028256, PubMed:8939742). Binds double-stranded target DNA, irrespective of the cytosine methylation status (). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia (PubMed:11100120, PubMed:15958557). Plays a role in the regulation of cell survival, proliferation and cell death (PubMed:15265859, PubMed:15958557). Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation (PubMed:15958557). Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy (PubMed:15265859). Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia (PubMed:11100120). Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (PubMed:8703054).