Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serum paraoxonase/arylesterase 2 (PON2) Standard Curve.)
Mouse Serum Paraoxonase/Arylesterase 2 (PON2) ELISA Kit | PON2 elisa kit
Mouse Serum Paraoxonase/Arylesterase 2 (PON2) ELISA Kit
Gene Names
Pon2; AI481612; 6330405I24Rik
Reactivity
Mouse
Synonyms
Serum Paraoxonase/Arylesterase 2 (PON2); Mouse Serum Paraoxonase/Arylesterase 2 (PON2) ELISA Kit; PON2; A-esterase 2; aromatic esterase 2; paraoxonase nirs; serum aryldialkylphosphatase 2; serum paraoxonase/arylesterase 2; PON2 elisa kit
Reactivity
Mouse
Specificity
Mouse Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PON2. No significant cross-reactivity or interference between Mouse PON2 and analogues was observed.
Sequence Length
354
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serum paraoxonase/arylesterase 2 (PON2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serum paraoxonase/arylesterase 2 (PON2) Standard Curve.)
Related Product Information for PON2 elisa kit
Description: This Mouse Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit employs a two-site sandwich ELISA to quantitate PON2 in samples. An antibody specific for PON2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PON2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PON2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PON2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Serum paraoxonase/arylesterase 2 is a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.
Background: Serum paraoxonase/arylesterase 2 is a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serum paraoxonase/arylesterase 2
NCBI Official Synonym Full Names
paraoxonase 2
NCBI Official Symbol
Pon2
NCBI Official Synonym Symbols
AI481612; 6330405I24Rik
NCBI Protein Information
serum paraoxonase/arylesterase 2
UniProt Protein Name
Serum paraoxonase/arylesterase 2
Protein Family
UniProt Gene Name
Pon2
UniProt Synonym Gene Names
PON 2; A-esterase 2
Uniprot Description
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters.
Research Articles on PON2
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Product Notes
The Mouse PON2 pon2 (Catalog #AAA9717322) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717322 ELISA Kit recognizes Mouse PON2. It is sometimes possible for the material contained within the vial of "Serum Paraoxonase/Arylesterase 2 (PON2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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