Mouse anti-Human IL-8 Monoclonal Antibody | anti-IL-8 antibody
Anti-Human IL-8 (I8-61) Antibody
Gene Names
CXCL8; IL8; AMCF-I
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Synonyms
IL-8; Monoclonal Antibody; Anti-Human IL-8 (I8-61) Antibody; Human IL-8; anti-IL-8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Specificity
This antibody recognizes both, recombinant and native human IL-8
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
103
Applicable Applications for anti-IL-8 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: React with human IL-8.
Neutralizing: In chemotaxis assay, the antibody inhibited 97% of the chemotactic activity of IL-8 on RB/293 cell using 5 Ug/ml of MAb and 10ng/ml of human IL-8 in assay. This MAb inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay. It indicates the MAb is special neutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in human granulocytes using FACS.
Neutralizing: In chemotaxis assay, the antibody inhibited 97% of the chemotactic activity of IL-8 on RB/293 cell using 5 Ug/ml of MAb and 10ng/ml of human IL-8 in assay. This MAb inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay. It indicates the MAb is special neutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in human granulocytes using FACS.
Immunogen
Purified recombinant human IL-8
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Related Product Information for anti-IL-8 antibody
Mouse monoclonal antibody to human Interleukin-8 (IL-8), Protein G affinity purified
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-8
NCBI Official Symbol
CXCL8
NCBI Official Synonym Symbols
IL8; AMCF-I
NCBI Protein Information
interleukin-8
UniProt Protein Name
Interleukin-8
UniProt Gene Name
IL8
UniProt Synonym Gene Names
CXCL8; IL-8; AMCF-I
UniProt Entry Name
IL8_PIG
Uniprot Description
Function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Subunit structure: Homodimer.
Subcellular location: Secreted.
Tissue specificity: Alveolar macrophages.
Induction: By lipopolysaccharide (LPS).
Post-translational modification: Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation
By similarity.
Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
Research Articles on IL-8
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Product Notes
The IL-8 il8 (Catalog #AAA4158331) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human IL-8 (I8-61) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: React with human IL-8. Neutralizing: In chemotaxis assay, the antibody inhibited 97% of the chemotactic activity of IL-8 on RB/293 cell using 5 Ug/ml of MAb and 10ng/ml of human IL-8 in assay. This MAb inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay. It indicates the MAb is special neutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in human granulocytes using FACS. Researchers should empirically determine the suitability of the IL-8 il8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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