Western Blot (WB)
(Western Blot analysis of various cells using Vimentin Polyclonal Antibody at dilution of 1:1000.)
Vimentin Antibody Kit
Vimentin Antibody Sampler Kit
Applications
Western Blot
Synonyms
Vimentin; Vimentin Antibody Sampler Kit; Vimentin antibody kit
Applicable Applications for Vimentin antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of various cells using Vimentin Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of various cells using Vimentin Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 293 cells with Phospho-Vimentin (Ser83) Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 293 cells with Phospho-Vimentin (Ser83) Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of A549 cells with Phospho-Vimentin (Ser56) Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of A549 cells with Phospho-Vimentin (Ser56) Polyclonal Antibody.)
Related Product Information for Vimentin antibody kit
Background: The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell-specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape. In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes. Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two. Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways. Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin. Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium. During mitosis, CDK1 phosphorylates vimentin at Ser56. This phosphorylation provides a PLK binding site for vimentin-PLK interaction. PLK further phosphorylates vimentin at Ser82, which might serve as memory phosphorylation site and play a regulatory role in vimentin filament disassembly. Additionally, studies using various soft-tissue sarcoma cells have shown that phosphorylation of vimentin at Ser39 by Akt1 enhances cell migration and survival, suggesting that vimentin could be a potential target for soft-tissue sarcoma targeted therapy.
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Product Notes
The Vimentin (Catalog #AAA2550086) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Vimentin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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