Typical Testing Data/Standard Curve (for reference only)
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Rat IL-8 ELISA Kit | IL-8 elisa kit
Rat IL-8 ELISA Kit
Gene Names
CXCL8; IL8; AMCF-I
Reactivity
Rat
Synonyms
IL-8; Rat IL-8 ELISA Kit; Rat Interleukin 8; IL-8 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for the detection of IL-8. No significant cross-reactivity or interference between IL-8 and any homologous proteins assayed has been observed. Cross-reactivity with other species has not been specifically determined.
Sequence Length
103
Samples
Serum, plasma, cell culture supernatants, tissue homogenate, body fluid
Assay Type
Quantitative Competitive
Sensitivity
~1.0pg/ml
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for IL-8 elisa kit
Principle of the Assay: The IL-8 ELISA is a quantitative competitive immunoassay. The plate provided is coated with IL-8 specific antibody. Standards and experimental samples are co-incubated in wells along with IL-8-HRP conjugate. IL-8 in standards and samples competes with the IL-8-HRP conjugate for binding to the plate-bound antibody. Higher levels of IL-8 from standards or samples lead to decreased IL-8-HRP conjugate binding and thus reduced signal. Captured IL-8-HRP is quantitatively detected by incubation with HRP substrates (substrates A and B). Binding of the IL-8-HRP is visualized by production of colorimetric reaction products that can be quantitatively measured by EMR absorbance at 450nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-8
NCBI Official Symbol
CXCL8
NCBI Official Synonym Symbols
IL8; AMCF-I
NCBI Protein Information
interleukin-8
UniProt Protein Name
Interleukin-8
UniProt Gene Name
IL8
UniProt Synonym Gene Names
CXCL8; IL-8; AMCF-I
UniProt Entry Name
IL8_PIG
Uniprot Description
Function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Subunit structure: Homodimer.
Subcellular location: Secreted.
Tissue specificity: Alveolar macrophages.
Induction: By lipopolysaccharide (LPS).
Post-translational modification: Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation
By similarity.
Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
Research Articles on IL-8
Similar Products
Product Notes
The Rat IL-8 il8 (Catalog #AAA9141543) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9141543 ELISA Kit recognizes Rat IL-8. It is sometimes possible for the material contained within the vial of "IL-8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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