Human IL-8 ELISA Kit | IL-8 elisa kit

IL-8 Human ELISA Kit

Cat. Num. AAA355339

• Gene Names: IL8; CXCL8; AMCF-I
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL-8; IL-8 Human ELISA Kit; Human IL-8; IL-8 elisa kit

• Reactivity: Human

• Applicable Applications for IL-8 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of IL-8 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Samples: Human serum, plasma, body fluids, tissue lysates or cell culture supernatants
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml (body fluids, tissue lysates or cell culture supernates) 7.8 pg/ml-500 pg/ml (human serum, plasma)
• Sensitivity: < 1 pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-8 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-8 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-8 can be calculated.

Background/Introduction: Interleukin-8 (IL8) is a member of the CXC chemokine family. It is proinflammatory and primarily mediates the activation and migration of neutrophils into tissue from peripheral blood. It produced by macrophages, and also synthesized by endothelial cells. IL-8 is one of the major mediators of the inflammatory response. It is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Primary function of IL-8 is the induction of chemotaxis in its target cells, and it also recruits neutrophils to phagocytose the antigen, which triggers the antigen pattern toll-like receptors. IL-8 is often associated with inflammation. As an example, it has been cited as a proinflammatory mediator in gingivitis and psoriasis.

NCBI and Uniprot Product Information

• NCBI GI #: 47523124
• NCBI GeneID: 396880
• NCBI Accession #: NP_999032.1
• NCBI GenBank Nucleotide #: NM_213867.1
• UniProt Accession #: P26894; P22951
• Molecular Weight: 11,633 Da
• NCBI Official Full Name: interleukin-8
• NCBI Official Symbol: IL8
• NCBI Official Synonym Symbols: CXCL8; AMCF-I
• NCBI Protein Information: interleukin-8; IL-8; C-X-C motif chemokine 8; alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I
• UniProt Protein Name: Interleukin-8
• UniProt Gene Name: IL8
• UniProt Synonym Gene Names: CXCL8; IL-8; AMCF-I
• UniProt Entry Name: IL8_PIG

Uniprot Description

Function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Alveolar macrophages.

Induction: By lipopolysaccharide (LPS).

Post-translational modification: Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation

By similarity.

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.