Western Blot (WB)
(Western Blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.)
Mouse anti-Human IL-31 Monoclonal Antibody | anti-IL-31 antibody
IL-31 Monoclonal Antibody
Gene Names
IL31; IL-31
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Protein affinity purified.
Synonyms
IL-31; Monoclonal Antibody; IL-31 Monoclonal Antibody; IL 31; IL31; Interleukin 31; Interleukin-31; anti-IL-31 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG3
Specificity
IL-31 monoclonal antibody detects endogenous levels of IL-31 protein.
Purity/Purification
Protein affinity purified.
Form/Format
Mouse IgG3, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
164
Applicable Applications for anti-IL-31 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
WB: 1:500
ICC: 1:100
IHC: 1:50-1:200
ICC: 1:100
IHC: 1:50-1:200
Immunogen
Recombinant full length protein corresponding to human IL18.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.)
Western Blot (WB)
(Western Blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.)
Immunocytochemistry (ICC)
(ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-IL-31 antibody
IL-31 is a T cell cytokine that is preferentially produced by T helper type 2 cells. IL-31 signals through a heterodimeric receptor composed of the IL-31 receptor (IL-31R) and the oncostatin M receptor (OSM). This receptor complex recruits JAK1, JAK2, Stat1, Stat3 and Stat5 signaling pathways, as well as the PI3 kinase/AKT cascade. SHP-2 and Shc adapter molecules are also recruited and contribute to an increased activation of the MAP kinase pathway in response to IL-31. Overexpression of IL-31 in mice results in pruritus and skin dermatitis resembling human atopic dermatitis (AD). Comparisons between skin from patients with AD and healthy skin showed IL-31R expression at higher levels on epidermal keratinocytes in AD samples. Infiltrating cells, more numerous in skin from patients with AD compared with that of healthy individuals, expressed IL-31 mRNA. IL-31 may participate in the cause of itch sensation and promote scratching behavior in NC/Nga mice with atopic dermatitis, and may represent a novel target for antipruritic drug development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18kDa
NCBI Official Full Name
interleukin-31
NCBI Official Synonym Full Names
interleukin 31
NCBI Official Symbol
IL31
NCBI Official Synonym Symbols
IL-31
NCBI Protein Information
interleukin-31
UniProt Protein Name
Interleukin-31
UniProt Gene Name
IL31
UniProt Synonym Gene Names
IL-31
UniProt Entry Name
IL31_HUMAN
NCBI Description
IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008]
Uniprot Description
IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
Protein type: Secreted; Cytokine; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: extracellular space
Molecular Function: cytokine activity
Biological Process: immune system process
Research Articles on IL-31
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Product Notes
The IL-31 il31 (Catalog #AAA3014483) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IL-31 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:500 ICC: 1:100 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the IL-31 il31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-31, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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