Bovine 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR/HMG-CoAR) ELISA Kit | HMGCR/HMG-CoAR elisa kit

Bovine 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMGCR/HMG-CoAR) ELISA Kit

Cat. Num. AAA2610306

• Gene Names: HMGCR; LDLCQ3
• Reactivity: Bovine
• Synonyms: 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR/HMG-CoAR); Bovine 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMGCR/HMG-CoAR) ELISA Kit; HMGCR/HMG-CoAR elisa kit

• Reactivity: Bovine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 835

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HMGCR/HMG-CoAR elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Bovine HMGCR/HMG-CoAR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 196049380
• NCBI GeneID: 3156
• NCBI GenBank Nucleotide #: NM_001130996.1
• Molecular Weight: 99,752 Da
• NCBI Official Full Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform 2
• NCBI Official Synonym Full Names: 3-hydroxy-3-methylglutaryl-CoA reductase
• NCBI Official Symbol: HMGCR
• NCBI Official Synonym Symbols: LDLCQ3
• NCBI Protein Information: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• UniProt Protein Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase
• UniProt Gene Name: HMGCR
• UniProt Synonym Gene Names: HMG-CoA reductase
• UniProt Entry Name: HMDH_HUMAN

NCBI Description

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: EC 1.1.1.34; Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Oxidoreductase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis

Chromosomal Location of Human Ortholog: 5q13.3-q14

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; peroxisomal membrane

Molecular Function: coenzyme binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; hydroxymethylglutaryl-CoA reductase activity; protein binding

Biological Process: cholesterol biosynthetic process; isoprenoid biosynthetic process; protein tetramerization