Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human IRF3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Interferon Regulatory Factor 3 Polyclonal Antibody | anti-IRF3 antibody
Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)
Immunohistochemistry: 5-20 ug/mL;1:36-146
Immunocytochemistry: 5-20 ug/mL;1:36-146
Optimal working dilutions must be determined by end user
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human IRF3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human IRF3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Hela cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Hela cells))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus
Molecular Function: DNA binding; identical protein binding; protein binding; protein domain specific binding; protein homodimerization activity; sequence-specific DNA binding; transcription cofactor activity
Biological Process: apoptosis; cellular response to DNA damage stimulus; cellular response to dsRNA; defense response to virus; lipopolysaccharide-mediated signaling pathway; macrophage apoptotic process; MDA-5 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of type I interferon production; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of transcription from RNA polymerase II promoter; programmed necrotic cell death; regulation of type I interferon production; response to exogenous dsRNA; transcription from RNA polymerase II promoter; type I interferon biosynthetic process; viral process
Disease: Herpes Simplex Encephalitis, Susceptibility To, 7
Research Articles on IRF3
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Product Notes
The IRF3 irf3 (Catalog #AAA2027197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon Regulatory Factor 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL;1:360-1460 Immunohistochemistry: 5-20 ug/mL;1:36-146 Immunocytochemistry: 5-20 ug/mL;1:36-146 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IRF3 irf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon Regulatory Factor 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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