Typical Testing Data/Standard Curve (for reference only)
Chicken Pyruvate Dehydrogenase Phosphatase ELISA Kit | PDP elisa kit
Chicken Pyruvate Dehydrogenase Phosphatase ELISA Kit
Gene Names
Pdp; 153332_at; CG12151; DmelCG12151; pdp; PDP
Reactivity
Chicken
Synonyms
Pyruvate Dehydrogenase Phosphatase; Chicken Pyruvate Dehydrogenase Phosphatase ELISA Kit; PDP elisa kit
Reactivity
Chicken
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 U/L
Preparation and Storage
Store all reagents at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PDP elisa kit
Intended Uses: This PDP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PDP. This ELISA kit for research use only!
Principle of the Assay: PDP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDP antibody and an PDP-HRP conjugate. The assay sample and buffer are incubated together with PDP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDP concentration since PDP from samples and PDP-HRP conjugate compete for the anti-PDP antibody binding site. Since the number of sites is limited, as more sites are occupied by PDP from the sample, fewer sites are left to bind PDP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PDP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDP antibody and an PDP-HRP conjugate. The assay sample and buffer are incubated together with PDP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDP concentration since PDP from samples and PDP-HRP conjugate compete for the anti-PDP antibody binding site. Since the number of sites is limited, as more sites are occupied by PDP from the sample, fewer sites are left to bind PDP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PDP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,551 Da
NCBI Official Full Name
pyruvate dehydrogenase phosphatase, isoform B
NCBI Official Synonym Full Names
Pyruvate dehydrogenase phosphatase
NCBI Official Symbol
Pdp
NCBI Official Synonym Symbols
153332_at; CG12151; DmelCG12151; pdp; PDP
NCBI Protein Information
CG12151 gene product from transcript CG12151-RB; CG12151-PA; CG12151-PB; Pdp-PA; Pdp-PB; pyruvate dehydrogenase phosphatase
UniProt Protein Name
GM14286p
Protein Family
UniProt Gene Name
Pdp
UniProt Entry Name
Q9W3Q1_DROME
Uniprot Description
Sequence similarities: Contains 1 PP2C-like domain. SAAS SAAS001932
Similar Products
Product Notes
The Chicken PDP pdp (Catalog #AAA741877) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741877 ELISA Kit recognizes Chicken PDP. It is sometimes possible for the material contained within the vial of "Pyruvate Dehydrogenase Phosphatase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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