Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: |Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using hSNF2H (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.)
Mouse anti-Human HSNF2H Monoclonal Antibody | anti-SMARCA5 antibody
HSNF2H (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 5, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin A5, Sucrose Nonfermenting Protein 2 Homolog, SMARCA5, SNF2H, WCRF135)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot: 0.5ug/ml
Immunocytochemistry: 1:500
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: |Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using hSNF2H (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.)
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: |Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using hSNF2H (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.)
Western Blot (WB)
(Western Blotting analysis: |Representative lot data. |HeLa cell lysate was probed with hSNF2H (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and visualized using a chemiluminescence detection system.|Arrow indicates hSNF2H (~130kD).)
Western Blot (WB)
(Western Blotting analysis: |Representative lot data. |HeLa cell lysate was probed with hSNF2H (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and visualized using a chemiluminescence detection system.|Arrow indicates hSNF2H (~130kD).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]
Uniprot Description
SMARCA5: an ubiquitous enzyme with ATP-dependent DNA helicase activity. Possesses intrinsic ATP-dependent nucleosome remodelling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin in vitro; this may require intact histone H4 tails. The catalytic subunit of the four known chromatin remodelling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. Belongs to the SNF2/RAD54 helicase family, ISWI subfamily.
Protein type: EC 3.6.4.-; Transcription factor; EC 3.6.1.-; Histone-binding; Helicase
Chromosomal Location of Human Ortholog: 4q31.1-q31.2
Cellular Component: nucleoplasm; chromatin silencing complex; RSF complex; nuclear replication fork; nucleolus; condensed chromosome; nucleus
Molecular Function: protein binding; DNA binding; histone binding; ATPase activity; nucleosome binding; helicase activity; ATP binding
Biological Process: chromatin silencing at rDNA; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; negative regulation of gene expression, epigenetic; double-strand break repair; transcription initiation; ATP-dependent chromatin remodeling; gene expression; regulation of gene expression, epigenetic; nucleosome positioning
Research Articles on SMARCA5
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Product Notes
The SMARCA5 smarca5 (Catalog #AAA6008832) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSNF2H (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 5, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin A5, Sucrose Nonfermenting Protein 2 Homolog, SMARCA5, SNF2H, WCRF135) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSNF2H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Western Blot and Immunocytochemistry. Dilution: Western Blot: 0.5ug/ml Immunocytochemistry: 1:500. Researchers should empirically determine the suitability of the SMARCA5 smarca5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSNF2H, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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