Mouse HOP, p60 Monoclonal Antibody
HOP, p60 (HSP70 HSP90 Organizing Protein)
Reactivity
Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
HOP; p60; Monoclonal Antibody; p60 (HSP70 HSP90 Organizing Protein); Anti -HOP; anti-HOP; p60 antibody
Host
Mouse
Reactivity
Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4E142
Specificity
Recognizes chicken HOP, p60. Species Crossreactivity: human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, mink, porcine, rabbit, sheep and Xenopus.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Applicable Applications for anti-HOP, p60 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Dilution: Western Blot (Colorimetric): 1:1000. Detects a band at ~60kD.
Optimal dilutions to be determined by researcher.
Optimal dilutions to be determined by researcher.
Immunogen
Chicken HOP (p60)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-HOP, p60 antibody
Hsp70-Hsp90 Organizing Protein (HOP, p60) is an ~60kD protein that is a critical intermediate component for the efficient maturation of steroid receptor complexes (1-3), serving to recruit Hsp90 to Hsp70-containing complexes. Unactivated steroid hormone receptors are found in hetero-oligomeric complexes that are thought to stabilize a partially folded receptor polypeptide prior to hormone-dependent activation (4-6). Hsp70 and Hsp90 are both known to associate with steroid hormone receptors. Numerous studies (8-13) have shown that Hsp70 functions in an ATP-dependent manner through transient interactions to mediate folding or unfolding of polypeptide chains. Hsp90 is thought to perhaps also function in some capacity related to folding or protein-protein interactions (14). HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. Hsp70 binding has been localized to TPR1, and Hsp90 binding has been localized to TPR2a. Importantly, the highly conserved ñEEVD sequence that terminates many Hsp70 family members, and the similar ñMEEVD sequence that terminates Hsp90, are important recognition sites for the TPR domains. The co-crystal structures for TPR1 plus a GPTIEEVD octapeptide (Hsp70 sequence) and TPR2a plus the MEEVD pentapeptide (Hsp90) have been solved (15). HOP is closely related to a human 63kD protein that is sensitive to simian virus SV 40 transformation (16) and is related to the yeast heat shock-responsive STI1 gene product (17).
Product Categories/Family for anti-HOP, p60 antibody
Similar Products
Product Notes
The HOP, p60 (Catalog #AAA604325) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HOP, p60 (HSP70 HSP90 Organizing Protein) reacts with Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's HOP, p60 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Dilution: Western Blot (Colorimetric): 1:1000. Detects a band at ~60kD. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the HOP, p60 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOP, p60, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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