Mouse Glucose Regulated Protein 75 Monoclonal Antibody

Glucose Regulated Protein 75 (Grp75)

Cat. Num. AAA605112

• Reactivity: Bovine, Canine, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: Glucose Regulated Protein 75; Monoclonal Antibody; Glucose Regulated Protein 75 (Grp75); Anti -Glucose Regulated Protein 75 (Grp75); anti-Glucose Regulated Protein 75 antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4E86
• Specificity: Detects a ~75kD protein, corresponding to the apparent molecular mass of Grp75 on SDS-PAGE. Species Crossreactivity: Human, monkey, mouse, rat, bovine, canine, chicken, Drosophila, guinea pig, hamster, porcine, rabbit, sheep and Xenopus.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 50% glycerol.

• Applicable Applications for anti-Glucose Regulated Protein 75 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot (Colorimetric): 1:1000. Sufficient for detection of Grp75 in 20ug of HeLa (heat shocked) cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:AP as the secondary antibody.; Immunohistochemistry: 1:50-1:200 using human breast cancer tissue.; Optimal dilutions to be determined by researcher.
• Immunogen: Grp75 isolated from human Molt-4 cells
• Positive Control: HeLa Heat Shocked Cell Lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB Analysis: Lane 1: MW marker; Lane 2: 20ug of HeLa cell lysate (heat shocked); Lane 3: 20ug of L-929 cell lysate; Lane 4: 20ug of Rat-2 cell lysate; Lane 5: 20ug of CHO-K1 cell lysate; Lane 6: 20ug of GPC-16 cell lysate. MBS605112 was used at 1:1000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human breast cancer tissue using MBS605112 .)

Related Product Information for anti-Glucose Regulated Protein 75 antibody

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the Hsp70 family, is comprised of multiple members, all of which bind ATP in vitro, but which are localized within different intracellular compartments. These include: i) Hsc70 (or constitutive form) present within the cytosol/nucleus; ii) Hsp70 (inducible form) present within the cytosol/nucleus/nucleolus; iii) the constitutive glucose-regulated 78kD (or BiP) protein present within the lumen of the endoplasmic reticulum; and iv) the constitutive glucose regulated 75kD protein present within the mitochondrial matrix. Members of the Hsp70 family are thought to funtion as molecular chaperones (2), assisting in the folding of other proteins in various intracellular compartments (3). Grp75 is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle (4). Like its E. coli homolog DnaK (5), Grp75 possesses a cation-dependent ATPase activity (6,7) thought to be central to its function as a chaperone.

Product Categories/Family for anti-Glucose Regulated Protein 75 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119692141
• NCBI Official Full Name: glucose regulated protein 75