Mouse Heat Shock Protein 70, Heat Shock Cognate Protein 70 Monoclonal Antibody | anti-HSP70 antibody

Heat Shock Protein 70, Heat Shock Cognate Protein 70 (HSP70, HSC70)

Cat. Num. AAA632744

• Gene Names: HSP70; grp78
• Reactivity: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Heat Shock Protein 70; Heat Shock Cognate Protein 70; Monoclonal Antibody; Heat Shock Cognate Protein 70 (HSP70; HSC70); Anti -Heat Shock Protein 70; anti-HSP70 antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4E106
• Specificity: Recognizes ~72 and ~73kD proteins, corresponding to the apparent molecular masses of human hsp70/hsc70. Species Crossreactivity: mouse, rat, beluga, bovine, canine, chicken, fish, guinea pig, hamster, monkey, porcine, plant, rabbit, sheep and Xenopus.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% azide, 50% glycerol.

• Applicable Applications for anti-HSP70 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Dilution: Western Blot (Colorimetric): 1:1000; Flow Cytometry: 1:100; Immunohistochemistry: Paraffin sections.; Optimal dilutions to be determined by researcher.; ; Positive Controls:; Human spleen tissue and human colon cancer Caco2 cells.
• Immunogen: Purified Hsc70 and Hsp70 isolated from human HeLa cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS632744: Lane 1: MW marker; Lane 2: HSC70/HSP73 (bovine), (recombinant); Lane 3: HSP70/HSP72 (human), (recombinant); Lane 4: 3T3; Lane 5: 3T3 (heat shocked); Lane 6: HeLa; Lane 7: HeLa (heat shocked); Lane 8: PC-12; Lane 9: PC-12 (heat shocked).)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of human colon cancer Caco2 cells analyzed by flow cytometry using isotype control.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of human spleen tissue stained with MBS632744 at 10ug/ml.)

Related Product Information for anti-HSP70 antibody

The heat shock protein, Hsp70 is part of the Hsp70 family which contains a number of highly-related protein isoforms ranging in size from 66kD to 78kD. The 70kD heat shock cognate protein, Hsp70 is closely related biochemically and biologically to Hsp70 and is part of the Hsp70 family. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an ATP-dependent manner. The molecular chaperones of the Hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. Recent data has demonstrated that BAG-1 which possesses a ubiquitin-like domain at its amino terminus and which has been shown to associate with the 26S proteosome in HeLa cells, modulates the chaperone activity of Hsc70 and Hsp70. There is also experimental data which shows that the ATPase domain and the substrate binding domain of Hsp70 (or Hsc70) cooperate and form a cochaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp) which is essential for normal neurotransmitter release.

Product Categories/Family for anti-HSP70 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 112984012
• NCBI GeneID: 692373
• NCBI Accession #: NP_001036837.1
• NCBI GenBank Nucleotide #: NM_001043372.1
• NCBI Official Full Name: heat shock 70 kD protein cognate
• NCBI Official Symbol: HSP70
• NCBI Official Synonym Symbols: grp78
• NCBI Protein Information: heat shock 70 kD protein cognate; heat shock protein 70; heat shock protein 70-3; glucose-regulated protein 78
• Protein Family: Heat shock 70 kDa protein