Testing Data
(Detection of Histone H3 phosphorylation in Jurkat cell lysate with anti-phospho-H3 mAb. Lane A. Untreated cell lysate; Lane BMBS633587 treated Jurkat cell lysate.)
Mouse anti-Human Histone H3 Monoclonal Antibody | anti-H3 antibody
Histone H3, phosphorylated (Ser28)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by protein G affinity chromatography.
Synonyms
Histone H3; Monoclonal Antibody; phosphorylated (Ser28); Anti -Histone H3; anti-H3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4H101
Specificity
Recognizes human Histone H3, phosphorylated (pSer28)
Purity/Purification
Affinity Purified
Purified by protein G affinity chromatography.
Purified by protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, before the addition of glycerol to 40%.
Applicable Applications for anti-H3 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 2ug/ml ~18kD band is detected only in treated cell lysate
Optimal dilution determined by the researcher.
Dilution: Western Blot: 2ug/ml ~18kD band is detected only in treated cell lysate
Optimal dilution determined by the researcher.
Immunogen
Synthetic peptide containing phosphorylated serine28 (CARKS*APATGGVKK) of human Histone H3.
Positive Control
Anti-Fas mAb treated Jurkat cell lysates.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Detection of Histone H3 phosphorylation in Jurkat cell lysate with anti-phospho-H3 mAb. Lane A. Untreated cell lysate; Lane BMBS633587 treated Jurkat cell lysate.)
Testing Data
(Detection of Histone H3 phosphorylation in Jurkat cell lysate with anti-phospho-H3 mAb. Lane A. Untreated cell lysate; Lane BMBS633587 treated Jurkat cell lysate.)
Related Product Information for anti-H3 antibody
The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, chromosome condensation and cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms. Metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
NCBI Protein Information
histone H3
Protein Family
Research Articles on H3
Similar Products
Product Notes
The H3 (Catalog #AAA633587) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, phosphorylated (Ser28) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 2ug/ml ~18kD band is detected only in treated cell lysate Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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