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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ODC1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 1.: ODC1 transfected 293T cell lysate)

Mouse anti-Human ODC1 Monoclonal Antibody | anti-ODC1 antibody

ODC1

Gene Names
ODC1; ODC
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
ODC1; Monoclonal Antibody; Ornithine decarboxylase; ODC; anti-ODC1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT9B3
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
461
Applicable Applications for anti-ODC1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Immunogen
Recombinant human ODC1 (1-461aa) purified from E. coli.
Clone Note
Anti-human ODC1 mAb, clone AT9B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ODC1 protein.
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ODC1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 1.: ODC1 transfected 293T cell lysate)

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ODC1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 1.: ODC1 transfected 293T cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of ODC1 in HeLa cells. The cell was stained with ATGA0507 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Immunofluorescence (IF) (ICC/IF analysis of ODC1 in HeLa cells. The cell was stained with ATGA0507 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Related Product Information for anti-ODC1 antibody
Ornithine decarboxylase, also known as ODC1, is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. ODC1 plays an important role in diverse biological processes, including cell growth, differentiation, transformation and apoptosis. Overexpression of the ODC1 gene plays important roles in cell proliferation and the development of cancer.
Product Categories/Family for anti-ODC1 antibody
References
Moshier J A., et al. (1992) Genomics. 1:77-86. Yang Feng T L., et al. (1996) Genomics. 36:449-458.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ornithine decarboxylase isoform 1
NCBI Official Synonym Full Names
ornithine decarboxylase 1
NCBI Official Symbol
ODC1
NCBI Official Synonym Symbols
ODC
NCBI Protein Information
ornithine decarboxylase
UniProt Protein Name
Ornithine decarboxylase
UniProt Gene Name
ODC1
UniProt Synonym Gene Names
ODC

NCBI Description

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2013]

Uniprot Description

Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.

Research Articles on ODC1

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Product Notes

The ODC1 odc1 (Catalog #AAA206623) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ODC1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ODC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the ODC1 odc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ODC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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