Rabbit anti-Human Hexokinase 3 Polyclonal Antibody | anti-HXK3 antibody

Hexokinase 3, CT (Hexokinase-3, HK3, HXK3, Hexokinase Type III, HK III, HKIII) (AP)

Cat. Num. AAA6480217

• Gene Names: HK3; HXK3; HKIII
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Hexokinase 3; Polyclonal Antibody; CT (Hexokinase-3; HK3; HXK3; Hexokinase Type III; HK III; HKIII) (AP); EC=2.7.1.1; Hexokinase 3 (White Cell); anti-HXK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HK3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HXK3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human HK3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-HK3 Pab is used in Western blot to detect HK3 in HL-60 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(HK3 Antibody (R775) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the HK3 antibody detected the HK3 protein (arrow).)

Product Categories/Family for anti-HXK3 antibody

Antibodies; Enzymes

References

1. Furuta, H., et al., Genomics 36(1):206-209 (1996). 2. Palma, F., et al., Mol. Cell. Biochem. 155(1):23-29 (1996). 3. Colosimo, A., et al., Cytogenet. Cell Genet. 74(3):187-188 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 194097330
• NCBI GeneID: 3101
• NCBI Accession #: NP_002106.2
• NCBI GenBank Nucleotide #: NM_002115.2
• UniProt Accession #: P52790; Q8N1E7
• Molecular Weight: 99,025 Da
• NCBI Official Full Name: hexokinase-3
• NCBI Official Synonym Full Names: hexokinase 3 (white cell)
• NCBI Official Symbol: HK3
• NCBI Official Synonym Symbols: HXK3; HKIII
• NCBI Protein Information: hexokinase-3; HK III; hexokinase type III; ATP:D-hexose 6-phosphotransferase
• UniProt Protein Name: Hexokinase-3
• UniProt Gene Name: HK3
• UniProt Synonym Gene Names: HK III
• UniProt Entry Name: HXK3_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]

Uniprot Description

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: EC 2.7.1.1; Carbohydrate Metabolism - starch and sucrose; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase; Enzyme, cellular metabolism; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: protein complex; mitochondrion; cytosol

Molecular Function: hexokinase activity; enzyme binding; mannokinase activity; hormone binding; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport