PLGF-2 elisa kit

Mouse PLGF-2 PicoKine ELISA Kit

Cat. Num. AAA1751078

• Gene Names: Pgf; PIGF; Plgf; AI854365
• Reactivity: Mouse; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: PLGF-2; Mouse PLGF-2 PicoKine ELISA Kit; Placenta growth factor; PlGF; Pgf; Plgf; PLGF-2 elisa kit

• Reactivity: Mouse; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Mouse PLGF-2
• Sequence Length: 158

• Applicable Applications for PLGF-2 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLGF-2 elisa kit

Principle of the Assay: Mouse PLGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLGF-2 has been precoated onto 96- well plates. Standards(Expression system for standard: sf21, Immunogen sequence: A24-P158) and test samples are added to the wells, a biotinylated detection biotinylated polyclonal antibody from goat specific for PLGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PLGF-2 amount of sample captured in plate.

Background: Placental growth factor is a protein that in humans is encoded by the PGF gene. Placental growth factor (PGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading maternal arteries. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal skeletal muscle. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 6679289
• NCBI GeneID: 18654
• NCBI Accession #: NP_032853.1
• NCBI GenBank Nucleotide #: NM_008827.3
• UniProt Accession #: P49764
• Molecular Weight: 17,876 Da
• NCBI Official Full Name: placenta growth factor isoform 1
• NCBI Official Synonym Full Names: placental growth factor
• NCBI Official Symbol: Pgf
• NCBI Official Synonym Symbols: PIGF; Plgf; AI854365
• NCBI Protein Information: placenta growth factor
• UniProt Protein Name: Placenta growth factor
• UniProt Gene Name: Pgf
• UniProt Synonym Gene Names: Plgf; PlGF

Uniprot Description

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth ().