Mouse anti-Human Hace1 Monoclonal Antibody | anti-Hace1 antibody
Hace1 Mouse Monoclonal Antibody (8D3)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Synonyms
Hace1; Monoclonal Antibody; Hace1 Mouse Monoclonal Antibody (8D3); E3 ubiquitin-protein ligase HACE1; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; HECT-type E3 ubiquitin transferase HACE1; KIAA1320; anti-Hace1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
8D3
Specificity
The antibody recognizes the full length HACE 1
Form/Format
Lyophilized protein G purified in PBS pH7.4
Sequence Length
194
Applicable Applications for anti-Hace1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
If reconstituted in 100 muL: WB: 1:500; IF: 1:500; IHC: 1:800.
Immunogen
Full length human Hacel recombinant protein
Preservative
None
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Hace1 antibody
HACE 1 (HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1) is an ubiquitin ligase with six N-terminal ankyrin and a C-terminal HECT domain. HACE 1 is ubiquitously expressed in normal tissues and is frequently downregulated in human tumors. HACE 1 has been reported to act as a tumor suppressor through a phosphorylation-dependent degradation of cyclin D1. HACE1 has also been shown to bind GTP-bound Rac1 (Ras-related C3 botulinum toxin substrate 1) and to catalyze its polyubiquitination. HACE1 expression increases the ubiquitination of Rac1 when the GTPase is activated. HACE1 is required for cytotoxic necrosis factor 1 (CNSFI)-mediated depletion of Rad and efficient invasion of endothelial cell monolayer by bacteria, which suggests a role for HACE1 in host defense against pathogens. More recently, HACE1 was reported to target the Golgi membrane through interactions with Rab proteins. During mitotic Golgi disassembly, the ubiquitin ligase activity of HACE1 is required for subsequent post-mitotic Golgi membrane fusion.
References
1. Zhang L-The E3 ligase HACE1 is a critical chromosome 6q21 tumor suppressor involved in multiple cancers.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
22,398 Da
NCBI Official Full Name
HACE1, partial
UniProt Protein Name
HACE1
Protein Family
UniProt Gene Name
HACE1
Uniprot Description
CautionLacks conserved residue(s) required for the propagation of feature annotation.
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Product Notes
The Hace1 hace1 (Catalog #AAA588012) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hace1 Mouse Monoclonal Antibody (8D3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hace1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). If reconstituted in 100 muL: WB: 1:500; IF: 1:500; IHC: 1:800. Researchers should empirically determine the suitability of the Hace1 hace1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hace1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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