Typical Testing Data/Standard Curve (for reference only)
(Mouse CD5L PicoKine ELISA Kit standard curve )
Mouse CD5L PicoKine ELISA Kit | Cd5l elisa kit
Mouse CD5L PicoKine ELISA Kit
Gene Names
Cd5l; 1/6; CT2; Pdp; Api6; mAIM; AAC-11; AI047839; Sp-alpha
Reactivity
Mouse
Synonyms
CD5L PicoKine; Mouse CD5L PicoKine ELISA Kit; CD5 antigen-like; AAC-11; AIM; API6; APOPTOSIS INHIBITOR 6; APOPTOSIS INHIBITOR OF MACROPHAGES; CD5 antigen like (scavenger receptor cysteine rich family); Cd5l; CD5L_HUMAN; CT 2; CT-2; Highly similar to ANTIGEN WC1.1 [Bos taurus]; IgM associated peptide; IgM-associated peptide; Pdp; PRO229; SCAVENGER RECEPTOR CYSTEINE-RICH FAMILY; SP ALPHA; SP-alpha; Spalpha; CD5 molecule-like; Cd5l elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD5L
Sequence Length
352
Assay Range
156pg/ml-10,000pg/ml
Sensitivity
<10pg/ml
Sample
For quantitative detection of mouse CD5L in cell culture supernates, serum and plasma(heparin, EDTA).
Immunogen
NSO,E22-V352
Assay Procedure Step 1
Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml mouse CD5L standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each mouse CD5L standard solution and each sample be measured in duplicate.
Intra-Assay Precision
Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|485|2021|8472|547|2381|8953
Standard deviation|20.37|70.74|432.07|284.44|145.24|644.62
CV(%)|4.2|3.5|5.1|5.2|6.1|7.2
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|485|2021|8472|547|2381|8953
Standard deviation|20.37|70.74|432.07|284.44|145.24|644.62
CV(%)|4.2|3.5|5.1|5.2|6.1|7.2
Sample Data
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10,000
O.D.|0.091|0.145|0.231|0.379|0.547|1.009|1.503|2.241
O.D.|0.091|0.145|0.231|0.379|0.547|1.009|1.503|2.241
Anticoagulant
Heparin or EDTA
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD5L PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD5L PicoKine ELISA Kit standard curve )
Related Product Information for Cd5l elisa kit
Principle of the assay: Mouse CD5L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD5L has been precoated onto 96-well plates. Standards(NSO, E22-V352) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD5L amount of sample captured in plate.
Background: CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.
Background: CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,863 Da
NCBI Official Full Name
CD5 antigen-like
NCBI Official Synonym Full Names
CD5 antigen-like
NCBI Official Symbol
Cd5l
NCBI Official Synonym Symbols
1/6; CT2; Pdp; Api6; mAIM; AAC-11; AI047839; Sp-alpha
NCBI Protein Information
CD5 antigen-like
UniProt Protein Name
CD5 antigen-like
Protein Family
UniProt Gene Name
Cd5l
UniProt Synonym Gene Names
; mAIM
UniProt Entry Name
CD5L_MOUSE
Uniprot Description
CD5L: May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis.
Protein type: Receptor, misc.; Secreted, signal peptide; Secreted
Cellular Component: cytoplasm; extracellular region; membrane
Molecular Function: scavenger receptor activity
Biological Process: apoptosis; immune system process; inflammatory response
Research Articles on Cd5l
Similar Products
Product Notes
The Mouse Cd5l cd5l (Catalog #AAA177650) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177650 ELISA Kit recognizes Mouse Cd5l. It is sometimes possible for the material contained within the vial of "CD5L PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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