Rabbit anti-Human, Mouse PRDX4 Polyclonal Antibody | anti-PRDX4 antibody

PRDX4, ID (PRDX4, Peroxiredoxin-4, Antioxidant enzyme AOE372, Peroxiredoxin IV, Thioredoxin peroxidase AO372, Thioredoxin-dependent peroxide reductase A0372) (FITC)

Cat. Num. AAA6337108

• Gene Names: PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRDX4; Polyclonal Antibody; ID (PRDX4; Peroxiredoxin-4; Antioxidant enzyme AOE372; Peroxiredoxin IV; Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372) (FITC); Thioredoxin-dependent peroxide reductase A0372; anti-PRDX4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PRDX4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PRDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the Central region of human PRDX4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PRDX4 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PRDX4 antibody detected the PRDX4 protein (arrow).)

Western Blot (WB)

(Western blot analysis of PRDX4 (arrow) using rabbit polyclonal PRDX4 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX4 gene.)

Western Blot (WB)

(PRDX4 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the PRDX4 antibody detected the PRDX4 protein (arrow).)

Immunohistochemistry (IHC)

(PRDX4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRDX4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PRDX4 antibody

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Product Categories/Family for anti-PRDX4 antibody

Antibodies; Enzymes; Peroxiredoxin

References

Jamaluddin, M., et al. J. Virol. 84(18):9533-9545(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Edvardsen, H., et al. Pharmacogenomics J. (2010). In press: Wang, H.Q., et al. FEBS Lett. 583(9):1511-1515(2009). Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5453549
• NCBI GeneID: 10549
• NCBI Accession #: NP_006397.1
• NCBI GenBank Nucleotide #: NM_006406.1
• UniProt Accession #: Q13162; Q6FHT3
• Molecular Weight: 30,540 Da
• NCBI Official Full Name: peroxiredoxin-4
• NCBI Official Synonym Full Names: peroxiredoxin 4
• NCBI Official Symbol: PRDX4
• NCBI Official Synonym Symbols: PRX-4; AOE372; AOE37-2; HEL-S-97n
• NCBI Protein Information: peroxiredoxin-4; antioxidant enzyme AOE372; epididymis secretory sperm binding protein Li 97n; peroxiredoxin IV; prx-IV; thioredoxin peroxidase (antioxidant enzyme); thioredoxin peroxidase AO372; thioredoxin-dependent peroxide reductase A0372
• UniProt Protein Name: Peroxiredoxin-4
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX4
• UniProt Synonym Gene Names: AOE37-2; Prx-IV
• UniProt Entry Name: PRDX4_HUMAN

NCBI Description

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDX4: Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. Belongs to the AhpC/TSA family.

Protein type: Secreted; Mitochondrial; Oxidoreductase; EC 1.11.1.15; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: extracellular space; smooth endoplasmic reticulum; mitochondrion; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; thioredoxin peroxidase activity

Biological Process: extracellular matrix organization and biogenesis; protein maturation via protein folding; 4-hydroxyproline metabolic process; cell redox homeostasis; male gonad development; spermatogenesis; I-kappaB phosphorylation