Western Blot (WB)
(Western Blot: Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells were probed with MBS601285 (0.5ug/ml).)
Mouse Hemagglutinin Tag Monoclonal Antibody
Hemagglutinin Tag (YPYDVPDYA) (HA Tag)
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Hemagglutinin Tag; Monoclonal Antibody; Hemagglutinin Tag (YPYDVPDYA) (HA Tag); Anti -Hemagglutinin Tag (YPYDVPDYA) (HA Tag); anti-Hemagglutinin Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG3
Clone Number
5H26
Specificity
Recognizes HA tagged (YPYDVPDYA) proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Applicable Applications for anti-Hemagglutinin Tag antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 0.5-2ug/ml detects a recombinant protein containing the HA-tag at the N-terminus in RIPA lysates from HEK293 cells.
Immunoprecipitation: 5ug/ml reported to immunoprecipitate recombinant HA-tagged fusion protein.
Immunocytochemistry: 5ug/ml reported to immunostain HA-tagged fusion protein.
Optimal dilutions to be determined by researhcer.
Dilution: Western Blot: 0.5-2ug/ml detects a recombinant protein containing the HA-tag at the N-terminus in RIPA lysates from HEK293 cells.
Immunoprecipitation: 5ug/ml reported to immunoprecipitate recombinant HA-tagged fusion protein.
Immunocytochemistry: 5ug/ml reported to immunostain HA-tagged fusion protein.
Optimal dilutions to be determined by researhcer.
Immunogen
Synthetic peptide corresponding to aa98-106 derived from the influenza virus hemaglutinin (HA) protein (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot: Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells were probed with MBS601285 (0.5ug/ml).)
Western Blot (WB)
(Western Blot: Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells were probed with MBS601285 (0.5ug/ml).)
Related Product Information for anti-Hemagglutinin Tag antibody
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Product Categories/Family for anti-Hemagglutinin Tag antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
hemagglutinin
Similar Products
Product Notes
The Hemagglutinin Tag (Catalog #AAA601285) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hemagglutinin Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Dilution: Western Blot: 0.5-2ug/ml detects a recombinant protein containing the HA-tag at the N-terminus in RIPA lysates from HEK293 cells. Immunoprecipitation: 5ug/ml reported to immunoprecipitate recombinant HA-tagged fusion protein. Immunocytochemistry: 5ug/ml reported to immunostain HA-tagged fusion protein. Optimal dilutions to be determined by researhcer. Researchers should empirically determine the suitability of the Hemagglutinin Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hemagglutinin Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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