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Western Blot (WB) (Western blot analysis of ASIC1 expression in 293T (A), mouse brain (B), rat brain (C) whole cell lysates.)

Rabbit ASIC1 Polyclonal Antibody | anti-ASIC1 antibody

Anti-ASIC1 Antibody

Gene Names
ASIC1; ASIC; ACCN2; BNaC2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
ASIC1; Polyclonal Antibody; Anti-ASIC1 Antibody; ACCN2; BNAC2; Acid-sensing ion channel 1; Amiloride-sensitive cation channel 2 neuronal; Brain sodium channel 2; BNaC2; anti-ASIC1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of ASIC1 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
528
Applicable Applications for anti-ASIC1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/50 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human ASIC1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of ASIC1 expression in 293T (A), mouse brain (B), rat brain (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of ASIC1 expression in 293T (A), mouse brain (B), rat brain (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of ASIC1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of ASIC1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-ASIC1 antibody
Rabbit polyclonal to ASIC1

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
41
UniProt Accession #
Molecular Weight
62,700 Da
NCBI Official Full Name
Acid-sensing ion channel 1
NCBI Official Synonym Full Names
acid sensing ion channel subunit 1
NCBI Official Symbol
ASIC1
NCBI Official Synonym Symbols
ASIC; ACCN2; BNaC2
NCBI Protein Information
acid-sensing ion channel 1
UniProt Protein Name
Acid-sensing ion channel 1
Protein Family
UniProt Gene Name
ASIC1
UniProt Synonym Gene Names
ACCN2; BNAC2; ASIC1; BNaC2
UniProt Entry Name
ASIC1_HUMAN

NCBI Description

This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.

Research Articles on ASIC1

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Product Notes

The ASIC1 asic1 (Catalog #AAA8242486) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ASIC1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ASIC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/50 - 1/200). Researchers should empirically determine the suitability of the ASIC1 asic1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASIC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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