Typical Testing Data/Standard Curve (for reference only)
Human B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L) ELISA Kit | Bcl9L elisa kit
Human B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L) ELISA Kit
Gene Names
BCL9L; BCL9-2; DLNB11
Reactivity
Human
Synonyms
B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L); Human B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L) ELISA Kit; DLNB11; B-cell lymphoma 9-like protein; Bcl9L elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L).
No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L) and analogues was observed.
No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L) and analogues was observed.
Sequence Length
1499
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.124ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Bcl9L elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for Bcl9L elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
152,547 Da
NCBI Official Full Name
B-cell CLL/lymphoma 9-like protein
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 9-like
NCBI Official Symbol
BCL9L
NCBI Official Synonym Symbols
BCL9-2; DLNB11
NCBI Protein Information
B-cell CLL/lymphoma 9-like protein
UniProt Protein Name
B-cell CLL/lymphoma 9-like protein
Protein Family
UniProt Gene Name
BCL9L
UniProt Synonym Gene Names
DLNB11; B-cell lymphoma 9-like protein; BCL9-like protein
UniProt Entry Name
BCL9L_HUMAN
Uniprot Description
Bcl-9L: a novel beta-catenin-interacting protein that translocates beta-catenin to the nucleus, enhancing beta-catenin mediated transcription. Can play an important role in colorectal carcinogenesis. Nuclear expression of Bcl-9L is closely associated with the high nuclear grade cancer phenotype and the expression of ErbB2/HER-2 in breast cancers.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: transcription coactivator activity; beta-catenin binding
Biological Process: transcription, DNA-dependent; somatic stem cell maintenance; regulation of cell morphogenesis; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; negative regulation of transforming growth factor beta receptor signaling pathway
Research Articles on Bcl9L
Similar Products
Product Notes
The Human Bcl9L bcl9l (Catalog #AAA2025599) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2025599 ELISA Kit recognizes Human Bcl9L. It is sometimes possible for the material contained within the vial of "B-Cell CLL/Lymphoma 9 Like Protein (Bcl9L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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