Immunoprecipitation (IP)
(PILRA was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-PILRA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PILRA rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa)
Rabbit anti-Human PILRA Polyclonal Antibody | anti-PILRA antibody
Anti-PILRA Antibody, Rabbit Polyclonal
ELISA: 1:25,000-1:50,000
IP: 1-2uL/mg of lysate
Immunoprecipitation (IP)
(PILRA was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-PILRA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PILRA rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa)
Immunoprecipitation (IP)
(PILRA was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-PILRA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PILRA rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa)
Western Blot (WB)
(Anti-PILRA rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:35 kDa)
Western Blot (WB)
(Anti-PILRA rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:35 kDa)
NCBI and Uniprot Product Information
NCBI Description
Cell signaling pathways rely on a dynamic interaction between activating and inhibiting processes. SHP-1-mediated dephosphorylation of protein tyrosine residues is central to the regulation of several cell signaling pathways. Two types of inhibitory receptor superfamily members are immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptors and their non-ITIM-bearing, activating counterparts. Control of cell signaling via SHP-1 is thought to occur through a balance between PILRalpha-mediated inhibition and PILRbeta-mediated activation. These paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This particular gene encodes the ITIM-bearing member of the receptor pair, which functions in the inhibitory role. Alternative splicing has been observed at this locus and three variants, each encoding a distinct isoform, are described. [provided by RefSeq, Jul 2008]
Research Articles on PILRA
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Product Notes
The PILRA (Catalog #AAA8107076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PILRA Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PILRA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the PILRA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PILRA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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