Human eNOS ELISA Kit | NOS3 elisa kit

Human eNOS ELISA Kit

Cat. Num. AAA8291345

• Gene Names: NOS3; eNOS; ECNOS
• Reactivity: Human
• Synonyms: eNOS; Human eNOS ELISA Kit; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3 elisa kit

• Reactivity: Human
• Specificity: The Human Endothelin 1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Endothelin 1 proteins within the range of 3.9 pg/ml-250 pg/ml.
• Sequence Length: 614

• Samples: Cell Culture Supernatants, Serum, Plasma
• Sensitivity: 0.5 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOS3 elisa kit

Principle of the Assay: The MyBioSource' Human Endothelin 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Endothelin 1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Endothelin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Endothelin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Endothelin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endothelin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1,-2, and-3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

NCBI and Uniprot Product Information

• NCBI GI #: 40254422
• NCBI GeneID: 4846
• NCBI Accession #: NP_000594.2
• NCBI GenBank Nucleotide #: NM_000603.4
• UniProt Accession #: P29474; Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2
• Molecular Weight: 67,876 Da
• NCBI Official Full Name: nitric oxide synthase, endothelial isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 3
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.