Rabbit anti-Human XRCC4 Polyclonal Antibody | anti-XRCC4 antibody

XRCC4, ID (XRCC4, DNA repair protein XRCC4, X-ray repair cross-complementing protein 4) (Biotin)

Cat. Num. AAA6364074

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: XRCC4; Polyclonal Antibody; ID (XRCC4; DNA repair protein XRCC4; X-ray repair cross-complementing protein 4) (Biotin); X-ray repair cross-complementing protein 4; anti-XRCC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-XRCC4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: XRCC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 204-234 amino acids selected from the Central region of human XRCC4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(XRCC4 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the XRCC4 antibody detected the XRCC4 protein (arrow).)

Related Product Information for anti-XRCC4 antibody

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq].

Product Categories/Family for anti-XRCC4 antibody

Antibodies; DNA Replication; Repair

References

Gomes, B.C., et al. Oncol. Rep. 24(4):1079-1085(2010). Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010). Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Liu, N., et al. Wei Sheng Yan Jiu 39(4):407-411(2010). Bau, D.T., et al. Anticancer Res. 30(7):2727-2730(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4507945
• NCBI GeneID: 7518
• NCBI Accession #: NP_003392.1
• NCBI GenBank Nucleotide #: NM_003401.3
• UniProt Accession #: Q13426; Q9BS72; Q9UP94; A8K3X4
• Molecular Weight: 38,287 Da
• NCBI Official Full Name: DNA repair protein XRCC4 isoform 1
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 4
• NCBI Official Symbol: XRCC4
• NCBI Protein Information: DNA repair protein XRCC4; X-ray repair cross-complementing protein 4; X-ray repair, complementing defective, repair in Chinese hamster
• UniProt Protein Name: DNA repair protein XRCC4
• Protein Family: Xrcc4-like factor
• UniProt Gene Name: XRCC4
• UniProt Entry Name: XRCC4_HUMAN

NCBI Description

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq, Sep 2008]

Uniprot Description

XRCC4: Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (XRCC6/XRCC5) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF. Widely expressed. Belongs to the XRCC4 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 5q14.2

Cellular Component: nucleoplasm; DNA ligase IV complex; centrosome; condensed chromosome; DNA-dependent protein kinase complex; cytosol; cell junction; nucleus

Molecular Function: protein C-terminus binding; protein binding; DNA binding; ligase activity

Biological Process: pro-B cell differentiation; positive regulation of neurogenesis; central nervous system development; viral reproduction; immunoglobulin V(D)J recombination; in utero embryonic development; T cell differentiation in the thymus; isotype switching; DNA repair; double-strand break repair via nonhomologous end joining; positive regulation of fibroblast proliferation; double-strand break repair; DNA ligation during DNA repair; response to gamma radiation; negative regulation of neuron apoptosis; positive regulation of ligase activity; response to X-ray

Disease: Short Stature, Microcephaly, And Endocrine Dysfunction