Mouse Golgi Marker, 58K Monoclonal Antibody

Golgi Marker, 58K

Cat. Num. AAA602047

• Reactivity: Porcine, Bovine, Monkey, Kangaroo, Rat, Hamster, Canine and Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Ascites
• Synonyms: Golgi Marker; 58K; Monoclonal Antibody; Anti -Golgi Marker; anti-Golgi Marker; 58K antibody

• Host: Mouse
• Reactivity: Porcine, Bovine, Monkey, Kangaroo, Rat, Hamster, Canine and Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3C140
• Specificity: Recognizes an epitope located on the rat microtubule-binding peripheral Golgi membrane 58K protein.
• Purity/Purification: Ascites
• Form/Format: Supplied as a liquid in 15mM sodium azide.

• Applicable Applications for anti-Golgi Marker, 58K antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and as a marker for the Golgi complex in Immunofluorescence.; Dilution: Western Blot: 1:5000-1:10,000; Immunofluorescence (IC): 1:50-1;100
• Immunogen: Golgi 58K full length from rat liver.
• Cellular Localization: Cytoplasmic and Golgi apparatus.
• Positive Control: Western Blot (Colometric): Whole rat liver extract.; Indirect Immunofluorescence:(CHO) cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot whole cell extract of Golgi membrane fraction from rat liver was separated on SDS-PAGE and blotted with MBS602047. The antibody was developed with Goat Anti-Mouse IgG (Fab Specific), Peroxidase Conjugate and AEC substrate. Lane A: Antibody dilution 1:10,000 Lane B: Negative control (only secondary antibody))

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells were fixed and permeabilized with 3% PFA plus 0.05% Triton X-100 in PBS. They were then stained with MBS602047 (1:50) followed by anti-mouse IgG conjugated to Cy3 (1:100). This picture was kindly supplied by Sarah Coleman.)

Immunofluorescence (IF)

(Immunofluorescence of Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD) antibody MBS602047)

Related Product Information for anti-Golgi Marker, 58K antibody

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Product Categories/Family for anti-Golgi Marker, 58K antibody

Antibodies; Abs to Golgi Proteins