Rabbit anti-Human 58K Golgi Polyclonal Antibody | anti-58K antibody

58K Golgi protein Polyclonal Antibody

Cat. Num. AAA3012178

• Gene Names: FTCD; LCHC1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: 58K Golgi; Polyclonal Antibody; 58K Golgi protein Polyclonal Antibody; FTCD; LCHC1; Formimidoyltetrahydrofolate cyclodeaminase; Formimidoyltransferase cyclodeaminase; Formiminotetrahydrofolate cyclodeaminase; Formiminotransferase cyclodeaminase; Formiminotransferase-cyclodeaminase; Glutamate formiminotransferase; Glutamate formyltransferase; LCHC 1; anti-58K antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: 58K Golgi protein polyclonal antibody detects endogenous levels of 58K Golgi protein protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 541

• Applicable Applications for anti-58K antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1,000; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-58K Golgi protein antibody. Counter stained with hematoxylin.)

Related Product Information for anti-58K antibody

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency [also known as formiminoglutamicaciduria (FIGLU-uria)], an autosomal recessive disorder. Features of a severe phenotype include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay and no hematological abnormalities.

NCBI and Uniprot Product Information

• NCBI GI #: 11140815
• NCBI GeneID: 10841
• NCBI Accession #: NP_006648.1
• NCBI GenBank Nucleotide #: NP_006648.1
• UniProt Accession #: O95954
• Molecular Weight: 59kDa
• NCBI Official Full Name: formimidoyltransferase-cyclodeaminase isoform A
• NCBI Official Synonym Full Names: formimidoyltransferase cyclodeaminase
• NCBI Official Symbol: FTCD
• NCBI Official Synonym Symbols: LCHC1
• NCBI Protein Information: formimidoyltransferase-cyclodeaminase
• UniProt Protein Name: Formimidoyltransferase-cyclodeaminase
• UniProt Gene Name: FTCD
• UniProt Synonym Gene Names: FTCD
• UniProt Entry Name: FTCD_HUMAN

NCBI Description

The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]

Uniprot Description

FTCD: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA); also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 4.3.1.4; EC 2.1.2.5; Lyase; Cofactor and Vitamin Metabolism - one carbon pool by folate; Amino Acid Metabolism - histidine

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: centriole; Golgi apparatus; cytoplasm; cytosol

Molecular Function: formimidoyltetrahydrofolate cyclodeaminase activity; glutamate formimidoyltransferase activity; folic acid binding

Biological Process: folic acid and derivative metabolic process; cytoskeleton organization and biogenesis; histidine catabolic process to glutamate and formamide; histidine catabolic process; histidine catabolic process to glutamate and formate

Disease: Formiminotransferase Deficiency