Testing Data
(Surface Staining of human peripheral blood with Mouse anti Human CD150:RPE )
Mouse anti-Human CD150 Monoclonal Antibody | anti-CD150 antibody
Mouse Anti Human CD150: RPE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Testing Data
(Surface Staining of human peripheral blood with Mouse anti Human CD150:RPE )
Testing Data
(Surface Staining of human peripheral blood with Mouse anti Human CD150:RPE )
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD3 and RPE conjugated mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD150 . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD3 and RPE conjugated mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD150 . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
CD150 is a membrane phosphoglycoprotein which contains 2 extracellular domains corresponding to a variable and constant region, a general feature of the Ig superfamily (Cocks et al. 1995). CD150 also has a cytoplasmic domain which contains 3 unique tyrosine motifs with a conserved TxYxxV sequence. This tyrosine motif is the binding domain for SLAM-associated protein (SAP) which form a complex together (Sayos et al 1998, Poy et al. 1999). This complex plays a role in the regulation of Natural killer (NK) and T cell development. Mutations to SAP in humans results in X-linked lymphoproliferative disease which is a fatal immunological disorder (Coffey et al. 1998). Activation of CD150 co-activates T- or B-lymphocytes responses and plays a role in bidirectional T-B cell stimulation (Carballido et al. 1997). It can also regulate NK and T cell development, B cell regulation and antibody production/isotype switching (Veillette 2006). CD150 is also the receptor that the measles virus recognizes (Tatsuo et al. 2000).
The SLAM.4 clone has been used in flow cytometry to analyze the cell distribution of CD150 on leucocytes and lymphocytes as well as to distinguish between subsets of CD4+ and CD8+ T lymphocytes (Romero et al. 2001).
NCBI and Uniprot Product Information
Uniprot Description
SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: integral to membrane; phagocytic vesicle; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; antigen binding
Biological Process: viral reproduction; regulation of catalytic activity; positive regulation of cell proliferation; regulation of vesicle fusion; lymphocyte activation; signal transduction
Research Articles on CD150
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Product Notes
The CD150 slamf1 (Catalog #AAA225759) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD150: RPE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD150 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Max. Dilution: Neat Use 10ul of the undiluted reagent to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the CD150 slamf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD150, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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